中药材2023，Vol.46Issue(6)：1439-1446,8.DOI:10.13863/j.issn1001-4454.2023.06.020
PMP柱前衍生化反应结合多元化学计量法和一测多评法的羊栖菜质量研究
Study on the Quality of Sargassum fusiforme Based on PMP Pre-column Derivatization Reaction Combined with Multivariate Chemometrics and QAMS
摘要
Abstract
Objective:To establish HPLC fingerprint and QAMS method for distinguishing Sargassum fusiforme decoction pieces from formula granules,and to screen key difference marker compounds,so as to provide reference for improving quality control standards of Sargassum fusiforme.Methods;According to the principle of precolumn derivatization of carbohydrate compounds,HPLC was used to determine the fingerprints of Sargassum fusiforme decoction pieces and formula granules.Systematic cluster analysis(HCA),principal component analysis(PC A)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to screen the difference markers.Using fucose as reference object,QAMS method was established for the determination of main monosaccharides in Sargassum fusiforme decoction pieces and formula granules.Results:The established fingerprint and QAMS method could effectively distinguish the decoction pieces and formula granules of Sargassum fusiforme.Fucose,D-galactose,D-glucose and two unknown compounds(peaks 5 and 10)were used as difference markers.However,the contents of D-mannose and fucose in formula granules were significantly differ-ent from those in decoction pieces.Conclusion:The fingerprint and four monosaccharide content determination method established in this study is stable and reliable,which can be used for quality control of Sargassum fusiforme decoction pieces and formula granules.关键词
羊栖菜/PMP柱前衍生化反应/指纹图谱/多元化学计量法/一测多评法Key words
Sargassum fusiforme(Harv.)Setch./PMP pre-column derivatization reaction/Fingerprint/Multivariate chemomet-rics/QAMS分类
医药卫生引用本文复制引用
瞿彩丽,谈小飞,黄茜茜,胡辉,龙林,成焕波,龚玲..PMP柱前衍生化反应结合多元化学计量法和一测多评法的羊栖菜质量研究[J].中药材,2023,46(6):1439-1446,8.基金项目
湖北省重点研发计划项目(2021ACA004,2020ACA019,2020ACA007-03) （2021ACA004,2020ACA019,2020ACA007-03）
湖北省光谷科创大走廊专项(2021BGE017) （2021BGE017）
