中药材2023，Vol.46Issue(12)：2923-2928,6.DOI:10.13863/j.issn1001-4454.2023.12.003
蚂蟥野生和人工养殖越冬亲本规模化繁殖研究
Reproduction Efficiency Analysis of Whitmania pigra Between the Wild and Artificial Overwintered Brood-Stock
摘要
Abstract
Objective:To analyze the reproduction efficiency of Whitmania pigra between the wild and artificial overwintered brood-stock,so as to provide scientific basis for large-scale reproduction of Whitmania pigra.Methods:Wild and artificial overwintered Whit-mania pigra collected in the spring were used as brood-stock samples,respectively.Using loam soil as the substrate,the artificial scale breeding experiment was carried out outdoors.The reproductive survival rate,body mass recovery rate,relative cocoon production rate,average number of cocoon production,cocoon size and cocoon hatching rate were counted.The effects of brood-stock density on repro-duction of overwintered Whitmania pigra were evaluated.Results:A total of 577.50 kg cocoons were collected from 2 644.83 kg wild Whitmania pigra in the density of 5.00 kg/m2,the cocoon rate,survival rate,body mass recovery rate,average number of cocoon pro-duction and cocoon hatching rate for wild Whitmania pigra were 21.84%,78.81%,42.74%,2.64 cocoon/per leech and 98.00%,re-spectively.The body mass recovery rate for overwintered Whitmania pigra in 4 different densities(3.87,4.97,6.07 and 7.13 kg/m2)were 30.40%,28.19%,30.84%and 28.69%,respectively.The survival rate in 4 different densities were 76.68%,74.03%,76.63%and 69.05%,respectively,and cocoon hatching rate was equal or greater than 98.00%.The body weight recovery rate,survival rate and cocoon hatching rate between densities were not significantly different.The average number of cocoon production in medium high density treatments was the highest(2.75 cocoon/per Whitmania pigra),which was significant difference between low and medium density treat-ments(P<0.05).Conclusion:There is litter difference of reproduction efficiency between the wild Whitmania pigra and overwintered Whitmania pigra.The artificial overwintered Whitmania pigra in early winter is low in price and reliable in quality,so they will be broad prospect in application for the Whitmania pigra artificial propagation.It is suggested that the breeding density of artificial breeding Whit-mania pigra should be controlled at about 6.10 kg/m2,and the survival rate and cocoon production effect are better.关键词
蚂蟥/规模化繁殖/亲本密度/繁殖效果Key words
Whitmania pigra Whitman/Large-scale reproduction/Brood-stock density/Reproduction efficiency分类
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王帅兵,宋扬,曹又文,熊良伟,王海华,徐亮..蚂蟥野生和人工养殖越冬亲本规模化繁殖研究[J].中药材,2023,46(12):2923-2928,6.基金项目
江西省重点研发计划项目(20212BBG73040) （20212BBG73040）
泰州市科技支撑计划(农业)项目(TN202303) （农业）
