中药材2023，Vol.46Issue(12)：2929-2937,9.DOI:10.13863/j.issn1001-4454.2023.12.004
茉莉酸甲酯调控牛大力有效成分积累及相关基因表达
Effects of Methyl Jasmonate on the Accumulation of Active Components and Related Gene Expressions in Callerya speciosa
摘要
Abstract
Objective:To study the effect of methyl jasmonate(MeJA)on the content of active components in Callerya speciosa,and explore the transcriptional regulation mode of MeJA on key enzyme genes in isoflavonoid biosynthesis and jasmonate(JA)signaling pathway.Methods:Using the three-month seedlings of Callerya speciosa as materials,the effect of MeJA on the contents of active compo-nents was analyzed by HPLC,transcriptome sequencing was performed by Illumina HiSeq platform,and the expressions of related genes were verified by qRT-PCR.Results:Exogenous MeJA increased the contents of index components in Callerya speciose,including form-ononetin and maackiain.Based on the transcriptome data of Callerya speciosa,a total of 20 727 differentially expressed genes(CDEGs)were found,of which 9 091 were up-regulated.KEGG enrichment analysis showed that DEGs were significantly enriched in plant hor-mone signal transduction,starch and sucrose metabolism,isoflavonoid biosynthesis etc.The qRT-PCR analysis confirmed the validity of the transcriptome data,indicating that the transcriptome data were reliable and accurate.Exogenous MeJA activated some key enzyme genes in isoflavonoid biosynthesis and JA signal pathway in Callerya speciosa.Among them,the flavonoid biosynthetic genes CHS,HIDs,I3'Hs,VR,PTR,CYP81E7,7IOMT and PTS and the genes involved in the JA signaling pathway,such as LOXs、AOSs、OPR、ACXs、JAZs and MYCs,were up-regulated.Conclusion:The contents of formononetin and maackiain are significantly increased through achieving by enhancing the expressions of LOX and MYC involved in JA signaling pathway and isoflavonoid biosynthetic genes CHS,HID-1 and VR response to exogenous MeJA in Callerya speciosa.关键词
牛大力/茉莉酸甲酯/基因表达/异黄酮/茉莉酸信号通路Key words
Callerya speciosa(Champ.ex Benth.)Schot/Methyl jasmonate/Gene expression/Isoflavonoids/Jjasmonate signaling pathway分类
医药卫生引用本文复制引用
余林婵,陈柳萍,覃春梅,明如宏,黄鼎,黄荣韶,姚绍嫦..茉莉酸甲酯调控牛大力有效成分积累及相关基因表达[J].中药材,2023,46(12):2929-2937,9.基金项目
国家自然科学基金项目(82060685,81760689) （82060685,81760689）
广西自然科学基金项目(2022GXNSFAA035497,2018GXNSFAA281287) （2022GXNSFAA035497,2018GXNSFAA281287）
广西壮瑶药重点实验室项目(20-065-14) （20-065-14）
