中医康复2024，Vol.1Issue(4)：6-10,17,6.DOI:10.19787/j.issn.2097-3128.2024.04.002
基于RNA-Seq技术分析经穴推拿对产后大鼠的生物学机制作用
Analysis of the Biological Mechanisms of Tuina on Postpartum Rats Based on RNA-Seq
李可 1梁笑语 1李一凡 1张皓淋 1官庆烨1
作者信息
摘要
Abstract
Objective:To investigate the effect of tuina on differential expression genes in postpartum rats by RNA sequencing(RNA-Seq)and ana-lyze the characteristics of biological mechanism.Methods:6 female SD rats were randomly divided into Control Pregnancy Group(CP)and Tuina Pregnancy Group(TP),with 3 in each group,then mated with male SD rats.Rats of TP were gived Tuina intervention for 7 days.RNA-Seq technique was used to detect the differential expression genes of blood,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis were performed for all rats.Results:Compared to CP,there were 959 differential expression genes in TP.GO analysis showed that the genes were mainly involved in metabolic,synthetic,and immune biological processes.KEGG analysis showed that the genes were primarily associated with the pathways of signal transduction,immune system,transport and catabolism,which related cell growth,differentiation,senescence,and apoptosis.Con-clusion:Tuina could regulate the gene expression in postpartum rats to participate in metabolism,immunity,and cell senescence,which provides evi-dence and support for Tuina adopting in postpartum functional recovery.关键词
产后/经穴推拿/RNA测序Key words
postpartum/tuina/RNA sequencing分类
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李可,梁笑语,李一凡,张皓淋,官庆烨..基于RNA-Seq技术分析经穴推拿对产后大鼠的生物学机制作用[J].中医康复,2024,1(4):6-10,17,6.
