草业学报2024，Vol.33Issue(3)：209-216,8.DOI:10.11686/cyxb2023210
质体ACCase Trp-1999-Leu突变对小麦田菵草生长适合度的影响
Fitness cost of the Trp-1999-Leu mutation in plastid ACCase on the growth of Beckmannia syzigachne
摘要
Abstract
In this study,we aimed to determine the fitness cost of the Trp-1999-Leu mutation in plastid acetyl co-enzyme A carboxylase(ACCase)on the growth of Beckmannia syzigachne,an important weed of cultivated wheat.The mutant had a homozygous mutation(Leu/Leu,RR)at the 1999 site of ACCase gene's carboxyl transferase(CT)region,compared with the wild type with Trp/Trp(SS)at the same site.The plant height,aboveground biomass in the early and vegetative stages of growth,fecundity at maturity,and competitiveness with wheat were compared between the wild type and the mutant to clarify the impact of the mutation on the growth of B.syzigachne without herbicide selection pressure.Pot experiments in a greenhouse were conducted to evaluate plant height and aboveground biomass in the early and vegetative stages of growth.The competitiveness of the wild type and the mutant was evaluated using both pot experiments in a greenhouse and field experiments.The results showed that the biomass and plant height of the RR genotype of B.syzigachne were 24.9%and 11.5%lower than that of the SS genotype during 10-15 days of early growth.At the vegetative growth stage,the relative growth rate and net assimilation rate of the RR genotype were 18.2%and 28.6%lower than those of the SS genotype,and the leaf area ratio of the RR genotype was 14.0%higher than that of the SS genotype.However,there was no significant difference in aboveground biomass between the RR and SS genotypes.At the mature stage,the RR genotype allocated less resources to reproductive organs,and the fecundity and total seed weight per plant of the RR genotype were 13.1%and 11.4%lower than their respective values in the SS genotype.In terms of competitiveness,as the density of wheat increased,the aboveground biomass and seed yield of the RR genotype were significantly lower than those of the SS genotype in the greenhouse experiments.This result was confirmed in the field experiment,where the RR genotype showed weaker interspecific competitiveness compared with the SS genotype.Therefore,the ACCase Trp-1999-Leu mutation has significant reproductive and competitive fitness costs in B.syzigachne.These results provide a theoretical basis for the management of this genotype of B.syzigachne.关键词
菵草/乙酰辅酶A羧化酶/Trp-1999-Leu/适合度Key words
Beckmannia syzigachne/acetyl coenzyme A carboxylase/Trp-1999-Leu/fitness cost引用本文复制引用
白霜,吕庆浩,李怡慧,刘国平,罗小勇,李凌绪..质体ACCase Trp-1999-Leu突变对小麦田菵草生长适合度的影响[J].草业学报,2024,33(3):209-216,8.基金项目
山东省自然科学基金项目(ZR2020QC136,ZR2019MC043)和青岛农业大学大学生科技创新计划(2022-2023)资助. （ZR2020QC136,ZR2019MC043）
