草业科学2024，Vol.41Issue(2)：284-296,13.DOI:10.11829/j.issn.1001-0629.2022-0866
鸟岛湿地土壤产甲烷菌群落对不同降水梯度的响应
Response of soil methanogenic bacterial communities to different precipitation gradients in the Bird Island Wetland
摘要
Abstract
Methanogenic bacteria play an important role in the process of wetland CH4 emission.Changes in precipitation patterns have and will continue to affect the structure and function of wetland ecosystems,affecting the process of wetland CH4 emission.In this study,the lakeside wetland of Bird Island in Qinghai Lake was taken as the research object,five precipitation gradients were set up,and the mcrA Amplifier sequencing technique was used to detect soil methanogenic bacteria in order to explore changes in their community characteristics in response to to different precipitation gradients.The results showed that the dominant methanogenic bacteria in the soil of Bird Island were Methanomicrobiales,Methanosarcinales,and Methanobacteriales.The prediction of the FAPROTAX function showed that hydrogen-trophic methanogens were dominant in soil,which inferred that H2/CO2 reduced to the main pathway of CH4 production in Bird Island soil.LEfSe analysis showed that five precipitation gradients coexisted with 31 different microflora,and the number of differential microflora was the most under the treatment of 50%precipitation,with 10 different microflora from the order to species level.Overall,the community diversity of methanogens in Bird Island soil was stable,but the community structure was sensitive to changes in precipitation,and the relative abundance of some microflora changed significantly(P<0.05).The results can provide some reference for predicting the influence of precipitation change on soil carbon cycle in BirdIsland wetland.关键词
高寒湿地/碳循环/气候变化/产甲烷菌/微生物群落/mcrA/青藏高原Key words
alpine wetlands/carbon cycle/climate change/methanogens/microbial community/mcrA/Qinghai-Tibet Plateau引用本文复制引用
王霞,陈克龙,杜岩功,章妮,车子涵,暴涵,王恒生..鸟岛湿地土壤产甲烷菌群落对不同降水梯度的响应[J].草业科学,2024,41(2):284-296,13.基金项目
青海湖入湖河流原位净化及河口湿地生态治理技术集成与示范项目(2022-QY-204) （2022-QY-204）
水位上升下青海湖湖滨带生态系统变化及其对碳循环影响研究项目(2023-ZJ-905T) （2023-ZJ-905T）
