草业科学2024，Vol.41Issue(2)：459-467,9.DOI:10.11829/j.issn.1001-0629.2023-0099
单宁酸对豌豆蚜谷胱甘肽-S-转移酶活性与基因表达的影响
Effect of tannic acid on glutathione-S-transferase activity and gene expression in Acyrthosiphon pisum
摘要
Abstract
In this study,we aimed to determine the effects of tannic acid(2 g·L-1)on glutathione-S-transferase(GST)activity and gene expression in pea aphids(Acyrthosiphon pisum).Specifically,we measured the changes in GST activity and GST gene expression in different tissues of pea aphids after 48 hours of tannic acid treatment.The results show that GST activity decreased following tannic acid(2 g·L-1)treatment.Conversely,GST gene expression was affected;compared to the control,seven GST genes were up-regulated in the head of pea aphids,of which ApGST104 and ApGSTX1 were upregulated approximately threefold.Moreover,ten GST genes were upregulated in the chest,of which ApGSTD6 was upregulated 6.4 times higher than the control.Ten GST genes were upregulated in the abdomen,of which ApGST101 and ApGST105 were upregulated approximately fivefold.Nine GST genes were upregulated in the midgut,of which ApGST105 was upregulated by 3.2 times.These results show that tannic acid affected GST gene expression in pea aphids,with some GST genes being significantly upregulated(P<0.05).This result indicates that GST genes may play an important role in the metabolism of tannic acid in pea aphids.关键词
豌豆蚜/解毒酶/植物次生物质/中肠/GST基因/组织解剖/实时荧光定量PCRKey words
Acyrthosiphon pisum/detoxifying enzyme/plant allelochemicals/midgut/GST gene/tissue anatomy/real-time fluorescent quantitative PCR引用本文复制引用
王继祖,刘磊,王森山,宋丽雯..单宁酸对豌豆蚜谷胱甘肽-S-转移酶活性与基因表达的影响[J].草业科学,2024,41(2):459-467,9.基金项目
甘肃省高等学校创新基金项目(2022A-060) （2022A-060）
甘肃农业大学公招博士科研启动项目(GAN-KYQD-2019-28) （GAN-KYQD-2019-28）
