草业科学2024，Vol.41Issue(2)：480-490,11.DOI:10.11829/j.issn.1001-0629.2022-0895
高寒地区菊芋青贮物料耐低温乳酸菌的筛选与鉴定
Identification of low-temperature-tolerant lactic acid bacteria in Helianthus tuberosus silage in alpine regions
摘要
Abstract
This study aimed to identify low temperature-tolerant lactic acid bacteria(LAB)that can improve the fermentation quality of Helianthus tuberosus silage in alpine regions.The H.tuberosus silage named'Qingyu No.2'was used as the material,and LAB was separated using the plate method.Physiological and biochemical characteristics were used to analyze the characteristics of the strains,and 16S rRNA sequencing was conducted to identify the strain species.The results showed that a total of 34 LAB were separated.Among them,13 LAB,including 11 homotypic fermented LAB and two heterotypic fermented LAB,were identified with low temperature tolerance by physiological and biochemical screening.It was found that the strain GN02 grew faster(OD = 2.63).GN10 was found to have a stronger acid-producing capacity(pH = 3.59)by comparing the acid production and growth capacity at low temperatures,and LAB XN25 combined the two characteristics.Nine strains of Lactiplantibacillus plantarum,one strain of L.pentosus,one strain of L.coryniformis,one strain of L.brevis,and one strain that could not be identified were detected through 16S rRNA sequencing at species level.The study found the potential low-temperature-tolerant LAB,one strain of L.plantarum,one strain of L.pentosus,and one strain of L.brevis suitable for the fermentation of H.tuberosussilage in alpine regions.关键词
高寒地区/菊芋/青贮/平板培养法/耐低温乳酸菌/16S rRNA测序/菌株鉴定Key words
alpine regions/Helianthus tuberosus/silage/plate culture method/low temperature-tolerant LAB/16S rRNA sequencing/strain identification引用本文复制引用
魏晓强,孙雪梅,张海旺,王丽慧,杨世鹏,司诚,钟启文,芦光新..高寒地区菊芋青贮物料耐低温乳酸菌的筛选与鉴定[J].草业科学,2024,41(2):480-490,11.基金项目
青海省重点研发项目(2022-NK-122) （2022-NK-122）
青海省自然科学基金项目(2022-ZJ-902) （2022-ZJ-902）
国家大宗蔬菜产业技术体系项目(CARS-23-G26) （CARS-23-G26）
