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复合型治沙措施对土壤细菌群落结构及功能的影响OA北大核心CSTPCD

Effects of compound sand control measures on soil bacterial community structure and function

中文摘要英文摘要

以流动沙丘地人工梭梭林土壤(Asd)为对照,选取尼龙网格沙障+人工梭梭林(Nn)和黏土沙障+人工梭梭林(Cy)2种复合型治沙措施区土壤为研究对象,采用Illumina高通量测序和PICRUSt2功能预测技术,探究复合型治沙措施对土壤细菌群落结构及功能的影响,并采用冗余分析解释影响土壤细菌群落及功能的主要理化因子.结果表明:1)2种复合型治沙措施区土壤细菌隶属于35门、90纲、172目、259科和436属.放线菌门、变形菌门和绿弯菌门是研究区土壤优势菌群,平均相对丰度累计达71.84%,其中放线菌门相对丰度最高,占32.16%~37.09%.Cy措施区土壤拟杆菌门和蓝藻菌门显著高于Asd样地(P<0.05).2)2种措施区土壤细菌Chao1指数显著高于流动沙丘土壤(P<0.05),但主坐标分析显示,3组样地土壤细菌群落结构组成相似.3)在17个次级功能中,碳水化合物代谢功能等为土壤细菌的主要生态功能,且在治沙措施区表现出高度冗余特点.4)相较流动沙丘土壤,复合型治沙措施区土壤有机质等养分含量呈显著增加的共性趋势(P<0.05),且冗余分析表明土壤有机质和速效钾等理化因子显著影响细菌群落结构及生态功能.5)土壤优势菌门与绝大多数代谢功能呈显著相关,且Mantel检验表明土壤细菌群落结构差异与功能潜势差异之间呈显著正相关.综上,复合型治沙措施的实施有助于改善荒漠土壤微环境,研究结果可为沙化土壤微环境生态修复提供理论依据.

In this research,the soil of artificial Haloxylon ammodendron forest(Asd)in mobile dune land was taken as the control,and soils from two other compound sand control systems[nylon grid sand barrier+artificial H.ammodendron forest(Nn)and clay sand barrier+artificial H.ammodendron forest(Cy)]were selected as experiment treatments.Illumina high-throughput sequencing and PICRUSt2 function prediction technology were used to explore the effects of the compound sand control measures on soil bacterial community structure and function,and redundancy analysis was used to explain the main physical and chemical factors affecting soil bacterial community structure and function.It was found that:1)The bacterial communities in soil samples from the two composite sand control measures comprised 35 phyla,90 classes,172 orders,259 families and 436 genera.Actinobacteria,Proteobacteria and Chloroflexi were the dominant soil bacterial taxa in the study area,with an average relative abundance of 71.84%.The relative abundance of Actinobacteria was the highest,accounting for 32.16%-37.09%.The occurrences of Bacteroidetes and Cyanobacteria in the Cy plot were significantly higher than those in the Asd plot(P<0.05).2)The Chao1 index of soil bacterial diversity in the two plots was significantly higher than that in the mobile dune soil(P<0.05),but the principal coordinate analysis showed that the soil bacterial community structure of the three plots was similar.3)Among the 17 secondary functions,carbohydrate metabolism and other functions were the main ecological functions of soil bacteria and showed highly redundant characteristics in different sand control measures areas.4)Compared with mobile dune soils,the soil organic matter and other nutrients in the area with composite sand control measures showed a common trend of significant increase in bacterial occurrence(P<0.05),and redundancy analysis showed that physicochemical factors such as soil organic matter and fast-acting potassium levels significantly affected the structure and ecological function of bacterial communities.5)The dominant phylum among the soil bacteria was significantly correlated with most metabolic functions,and the Mantel test showed a significant positive correlation between differences in soil bacterial community structure and differences in functional potential.In conclusion,the implementation of composite sand control measures improved the desert soil microenvironment,and the research results provide a theoretical basis to manage the ecological restoration of the sandy soil microenvironment.

王安林;马瑞;马彦军;刘腾;田永胜;董正虎;柴巧弟

甘肃农业大学林学院,甘肃 兰州 730070

土壤细菌群落结构治沙措施沙化土地修复

soil bacteriacommunity structuresand control measuressandy land restoration

《草业学报》 2024 (003)

46-60 / 15

国家自然科学基金区域创新发展联合基金项目(U21A2001)和甘肃农业大学青年导师扶持基金项目(GAU-QDFC-2020-09)资助.

10.11686/cyxb2023140

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