复合型治沙措施对土壤细菌群落结构及功能的影响OA北大核心CSTPCD

In this research,the soil of artificial Haloxylon ammodendron forest(Asd)in mobile dune land was taken as the control,and soils from two other compound sand control systems[nylon grid sand barrier+artificial H.ammodendron forest(Nn)and clay sand barrier+artificial H.ammodendron forest(Cy)]were selected as experiment treatments.Illumina high-throughput sequencing and PICRUSt2 function prediction technology were used to explore the effects of the compound sand control measures on soil bacterial community structure and function,and redundancy analysis was used to explain the main physical and chemical factors affecting soil bacterial community structure and function.It was found that:1)The bacterial communities in soil samples from the two composite sand control measures comprised 35 phyla,90 classes,172 orders,259 families and 436 genera.Actinobacteria,Proteobacteria and Chloroflexi were the dominant soil bacterial taxa in the study area,with an average relative abundance of 71.84%.The relative abundance of Actinobacteria was the highest,accounting for 32.16%-37.09%.The occurrences of Bacteroidetes and Cyanobacteria in the Cy plot were significantly higher than those in the Asd plot(P<0.05).2)The Chao1 index of soil bacterial diversity in the two plots was significantly higher than that in the mobile dune soil(P<0.05),but the principal coordinate analysis showed that the soil bacterial community structure of the three plots was similar.3)Among the 17 secondary functions,carbohydrate metabolism and other functions were the main ecological functions of soil bacteria and showed highly redundant characteristics in different sand control measures areas.4)Compared with mobile dune soils,the soil organic matter and other nutrients in the area with composite sand control measures showed a common trend of significant increase in bacterial occurrence(P<0.05),and redundancy analysis showed that physicochemical factors such as soil organic matter and fast-acting potassium levels significantly affected the structure and ecological function of bacterial communities.5)The dominant phylum among the soil bacteria was significantly correlated with most metabolic functions,and the Mantel test showed a significant positive correlation between differences in soil bacterial community structure and differences in functional potential.In conclusion,the implementation of composite sand control measures improved the desert soil microenvironment,and the research results provide a theoretical basis to manage the ecological restoration of the sandy soil microenvironment.