紫叶风箱果叶绿体基因组特征及绣线菊亚科系统发育分析OA北大核心CSTPCD

Physocarpus opulifolius'Diabolo'is an ornamental flowering shrub that has been introduced into China from North America in recent years.The aims of this study were to determine the structural characteristics of the chloroplast genome of P.opulifolius'Diabolo'and to clarify its taxonomic status within the subfamily Spiraeoideae.The chloroplast genome of P.opulifolius'Diabolo'was sequenced on the Illumina NovaSeq 6000 sequencing platform,and the complete chloroplast genome was assembled and annotated.The chloroplast genome of P.opulifolius'Diabolo'had a typical quadripartite structure,with a total length of 159131 bp,large single copy(LSC)length of 87582 bp,small single copy(SSC)length of 18829 bp,and inverted repeats a(IRa)and inverted repeats b(IRb)length of 26360 bp.A total of 130 genes were annotated,including 83 protein-coding genes,8 ribosomal RNA(rRNA)genes,37 transfer RNA(tRNA)genes,and 2 pseudogenes.Twelve codons(UUG,AUU,GUU,GUA,UAA,AAA,UCU,UCC,CCU,ACU,GCU,GGU)were determined.The codon usage bias was mainly affected by natural selection,and mutation was a weak influencing factor.Twenty-seven pairs of long sequence repeats and 87 simple sequence repeat loci were detected.The genes with high nucleotide polymorphism in the chloroplast genome of Physocarpus opulifolius'Diabolo'were psbZ,trnG(UCC),trnfM(CAU),trnH(GUG),psbA,rbcL,rpl20 and rps12.The results of the phylogenetic analysis showed that Physocarpus is closely related to the genus Neillia.