分析化学2024，Vol.52Issue(2)：178-186,中插1-中插3,12.DOI:10.19756/j.issn.0253-3820.231287
环境水体中氨氮微型快速检测装置的研制及应用
Development and Application of a Micro-device for Rapid Detection of Ammonia Nitrogen in Environmental Water
摘要
Abstract
The analysis of ammonia nitrogen in real water samples is challenging due to matrix interferences and difficulties for rapid on-site analysis.On the basis of the standard method,i.e.water quality-determination of ammonia nitrogen-salicylic acid spectrophotometry(HJ 536-2009),a simple device for online detecting ammonia nitrogen was developed using a sequential injection analysis(SIA)system in this work.The ammonia nitrogen transformation system,color reaction system,and detection system were built in compatible with the SIA system,respectively.In particular,the detection system was assembled by employing light-emitting diode as the light source,photodiode as the detector,and polyvinylchloride tube as the cuvette,thus significantly reducing the volume,energy consumption and fabricating cost of the detection system.As a result,the accurate analysis of ammonia nitrogen in complex water samples was achieved.A quantitative detection of ammonia nitrogen in water sample was obtained in 12 min,along with linear range extending to 1000 μmol/L,precisions(Relative standard deviation,RSD)of 4.3%(C=10 μmol/L,n=7)and 4.2%(C=500 μmol/L,n=7),and limit of detection(LOD)of 0.65 μmol/L(S/N=3,n=7).The results of interfering experiments showed that the detection of ammonia nitrogen by the developed device was not interfered by the common coexisting ions and components,therefore the environmental water could be directly analyzed,such as reservoir water,domestic sewage,sea water and leachate of waste landfill.The analytical results were consistent with those obtained by the environmental protection standard method(Water quality determination of ammonia nitrogen-salicylic acid spectrophotometry,HJ 536-2009).In addition,the spiking recoveries were in the range of 92.3%-98.1%,further confirming the accuracy and practicality of the developed device.关键词
氨氮/顺序注射/水杨酸分光光度法/在线检测/发光二极管Key words
Ammonia nitrogen/Sequential injection/Salicylic acid spectrophotometry/On-line detection/Light-emitting diode引用本文复制引用
王朋,田永,刘传禹,王炜亮,陈旭伟,张岩峰,陈明丽,王建华..环境水体中氨氮微型快速检测装置的研制及应用[J].分析化学,2024,52(2):178-186,中插1-中插3,12.基金项目
国家自然科学基金项目(No.22176106)、泰山学者工程专项项目(No.tsqn202306233)、山东省高等学校"青创人才引育计划"项目(2021)和智能警务四川省重点实验室开放课题项目(No.ZNJW2024KFZD005)资助.Supported by the National Natural Science Foundation of China(No.22176106),the Taishan Scholars Program of Shandong Province(No.tsqn202306233),the Shandong Province Higher Educational Program for Introduction and Cultivation of Young Innovative Talents(2021)and the Opening Project of Intelligent Policing Key Laboratory of Sichuan Province,China.(No.ZNJW2024KFZD005). （No.22176106）
