分析化学2024，Vol.52Issue(2)：198-207,10.DOI:10.19756/j.issn.0253-3820.231419
颜色编码液滴阵列的机器学习解码及其在多重数字化核酸分析中的应用
Machine Learning Enabled Decoding of Color-coded Droplet Arrays and Its Application in Multiplex Digital Nucleic Acid Analysis
摘要
Abstract
To address the throughput limitations of digital nucleic acid analysis,a tricolor combination-based droplet coding technique was developed to achieve multiplex digital nucleic acid analysis with flexible throughput expansibility.To improve the analysis efficiency,a machine learning-based method was further developed for automatic decoding of color-coded droplet array.The machine learning algorithm empowered the computer program to automatically extract the color-position-quantity information of the droplets.By correlating this color-position-quantity of droplets before and after nucleic acid amplification,the proportion of positive droplets for each target was rapidly determined.This droplet decoding strategy was applied to multiplex digital nucleic acid analysis.The experimental results demonstrated that this droplet decoding method was fast and accurate,with a decoding process completed within 2 min.Furthermore,the droplet identification accuracy exceeded 99%.Additionally,the obtained nucleic acid quantification results exhibited a good correlation(R2＞0.99)with those reported by a commercial digital PCR instrument.关键词
机器学习/液滴/编码/解码/多重数字化核酸分析Key words
Machine learning/Droplet/Coding/Decoding/Multiplex digital nucleic acid analysis引用本文复制引用
刘云帆,蔡东洋..颜色编码液滴阵列的机器学习解码及其在多重数字化核酸分析中的应用[J].分析化学,2024,52(2):198-207,10.基金项目
广东省自然科学基金项目(No.2021A1515010182)资助.Supported by the Natural Science Foundation of Guangdong Province,China(No.2021A1515010182). （No.2021A1515010182）
