分析化学2024，Vol.52Issue(2)：277-285,9.DOI:10.19756/j.issn.0253-3820.221336
基于磁固相萃取-自动前处理分离和富集动物肝脏中的β-受体激动剂
Separation and Enrichment of β-Agonists from Animal Livers Based on Magnetic Solid-Phase Extraction with Automated-treatment Device
摘要
Abstract
A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for determination of three kinds of β-agonists(Clenbuterol(CL),Ractopamine(RAC)and Salbutamol(SAL))residues in animal liver samples.The liver sample homogenates were extracted with organic solvent,followed by clean-up using the automatic magnetic solid-phase extraction(MSPE),and then analyzed using LC-MS/MS.The results showed that the magnetic mixed-mode cation exchange adsorbent(M-MCX)exhibited 34%higher adsorption capacity than the conventional mixed-mode cation exchange(MCX)column.Furthermore,the clean-up was conducted by using an automatic MSPE device,and 8 samples could be simultaneously treated within 30 min.The limits of detection(LOD)were 0.01-0.1 μg/kg,the average recoveries ranged from 88.2%to 110.5%,and the relative standard deviations(RSDs)were in range of 2.9%-10.3%at three spiked levels for the three kinds of β-agonists.Compared with the traditional SPE technique,the present method had many advantages such as simple operation,rapidity and high efficiency,which was suitable for high-throughput and automatic detection of residues in routine analysis.关键词
磁固相萃取/自动前处理/β-受体激动剂/肝脏/质谱Key words
Magnetic solid-phase extraction/Automatic pretreatment/β-Agonist/Liver/Mass spectrometry引用本文复制引用
魏书林,王紫豪,李桐,朱怀恩,单吉浩,宋志超,王瑞国..基于磁固相萃取-自动前处理分离和富集动物肝脏中的β-受体激动剂[J].分析化学,2024,52(2):277-285,9.基金项目
北京市科技计划项目(No.Z211100007021001)、中国农业科学院创新工程项目(饲料质量安全检测与评价)和国家现代农业产业技术体系项目(No.CARS-35)资助.Supported by the Beijing Municipal Science and Technology Project(No.Z211100007021001),the Innovation Program of the Chinese Academy of Agricultural Science(Feed Quality and Safety)and the China Agriculture Research System(No.CARS-35). （No.Z211100007021001）
