赣南医学院学报2024，Vol.44Issue(1)：9-14,6.DOI:10.3969/j.issn.1001-5779.2024.01.002
染料木素磺酸钠减轻慢性脑缺血大鼠神经细胞焦亡的研究
The effects of Genistein-3'-sodium sulfonate on the cortex of rats with chronic cerebral ischemia by inhibiting pyroptosis
摘要
Abstract
Objective:To investigate the effect of Genistein-3'-sodium sulfonate(GSS)on cell pyroptosis in rats with chronic cerebral ischemia.Methods:A modified bilateral common carotid artery occlusion(2-VO)method was used to establish a rat model of chronic cerebral ischemia.The rats were randomly divided into sham group(Sham),model group(2-VO+Vehicle)and treatment group(2-VO+GSS).IL-1β activity in rat serum was assessed using ELISA.The mRNA and protein expression levels of IL-1β,NLRP3,Caspase-1,and GSDMD in the cortical tissue of rats were detected by real-time PCR and Western blot.Results:Compared with the sham group,the activity of IL-1β in model group rats'serum decreased significantly(P=0.042 9),compared with model group,the activity of IL-1β in treatment group rats'serum decreased,but with no significant difference(P=0.073 3).The study of the mRNA or protein levels of IL-1β from the cerebral cortex showed,compared with sham group,the IL-1βmRNA and protein expression were increased(P=0.025 5,P＜0.000 1);compared with model group,the IL-1β mRNA and protein expression in the treatment group were decreased,with statistical significance(P=0.020 0,P＜0.000 1).Compared with sham group,the mRNA levelof NLRP3、GSDMD and Caspase-1 P20(P=0.044 3,P=0.022 9,P＜0.000 1)and protein level(P=0.000 3,P=0.000 7,P=0.074 1)in the cerebral cortex of all rats in the model group increased.Compared with model group,the mRNA level of NLRP3、GSDMDand Caspase-1 P20(P=0.001 2,P=0.004 4,P=0.005 8)and protein level(P=0.008 4,P=0.026 8,P=0.038 5)in the cerebral cortex of all rats in the treatment group were decreased.Conclusion:GSS may alleviate chronic cerebral ischemic injury by inhibiting neuronal pyroptosis.关键词
慢性脑缺血/染料木素磺酸钠/细胞焦亡Key words
Chronic cerebral ischemia/Genistein-3'-sodium sulfonate/Pyroptosis分类
医药卫生引用本文复制引用
邢亚康,张丽梅,王恺迪,温亚娟,黎晓,帅萍..染料木素磺酸钠减轻慢性脑缺血大鼠神经细胞焦亡的研究[J].赣南医学院学报,2024,44(1):9-14,6.基金项目
国家自然科学基金项目(82160688) （82160688）
赣州市指导性科技计划项目(GZ2021ZSF018,2022B-SF9554) （GZ2021ZSF018,2022B-SF9554）
赣南医学院本科生科技创新项目(202010) （202010）
赣南医学院神经生物研究创新团队项目(TD2021JC06) （TD2021JC06）
