赣南医学院学报2024，Vol.44Issue(1)：15-21,7.DOI:10.3969/j.issn.1001-5779.2024.01.003
"补肾填精"中药复方改善脑瘫大鼠运动发育落后的机制研究
Study on the mechanism of traditional Chinese medicine compound"Bu Shen Tian Jing"to improve motor development retardation in rats with cerebral palsy
摘要
Abstract
Objective:To explore the molecular mechanism of"Bu Shen Tian Jing"compound of traditional Chinese medicine to improve motor development in cerebral palsy(CP)of children.Methods:The cerebral palsy(CP)animal model was established in pregnant Wistar rats at 16 days of gestation by intraperitoneal injection of bacterial lipopolysac-charide(LPS)combined with a treatment of hypoxic living environment(8%oxygen + 92%nitrogen)for 2 h.The offspring CP rats were screened by neurobehavioral testing.The CP young mice and healthy mice were set up as treatment group and control group,respectively,with a total of 4 groups and 10 animals in each group.Another 10 normal young mice were taken as a blank control group without any treatment.The''Bu Shen Tian Jing''decoction-free granules was dissolved in water at a mass-to-volume ratio of 1 g·(25 mL)-1.Animals were treated with medicine or aqueous solvents by intragastric administration with a dosage of 5 mL·kg-1 body weight once a day for 15 days.The exercise capacity of rats was observed and evaluated on the 5th,10th and 15th days after administration,respectively.At the end of the animal experiment,the rats were sacrificed and the peripheral blood was collected in eyes socket.The inflammatory factors in the peripheral blood were detected by ELISA assay,and protein levels associated with the mTOR signaling pathway in the brain tissue were detected by western blotting(WB).Results:Compared with the blank control group,the suspension time of the CP control group was significantly shortened(P＜0.001);compared with the CP control group,the suspension time of the CP treatment group was significantly longer(P＜0.001),while in normal group,the suspension time in the treatment group and control group had no significant differences(P=0.064).The results from the slope experiment showed that compared with the blank control group,the head turning time of the CP control group was significantly longer,which had a significant difference(P＜0.001);Compared with the CP control group,the head turning time of the CP treatment group was significantly shortened,which had a statistically significant difference(P＜0.001).However,there was no statistically significant difference in normal group in treatment group and control group(P=0.863).The body weight of rats in each group was monitored every day.It was demonstrated that the body weight in normal treatment group had no significant difference compared with the blank control and normal control group(P＞0.05),while compared with the blank control group,the CP treatment group had a significant weight loss(P＜0.001).And CP treatment group had a significant increase in body weight compared with the CP control group(P=0.031).ELISA testing was used to detect the expression level of inflammatory factors in the peripheral blood of each group of animals.Compared with the CP control group,the pro-inflammatory factors TNF-α and IL-1β in the CP treatment group were significantly decreased(P＜0.001),with IL-6 had not significant(P＞0.05);while the anti-inflammatory factors IL-4 and IL-10 were significantly increased(P＜0.001).WB was used to detect the protein expression level associated with the PI3K/ATK/mTOR signaling pathway in the brain tissue.It was demonstrated that compared with the CP control group,the expression levels of PI3K,phosphorylated-AKT,phosphorylated-mTOR and autophagy-related protein p62 were increased in the CP treatment group;with the LC3-Ⅱ/Ⅰratio decrease(P＜0.01).Conclusion:The"Bu Shen Tian Jing"compound can improve the motor function of young rats with CP by regulating the mTOR signaling pathway-mediated autophagy of nerve cells and inhibiting the occurrence of inflammation.关键词
脑瘫/中药复方/自噬/炎症损伤Key words
Cerebral palsy/Traditional Chinese medicine compound/Autophagy/Inflammatory damage分类
医药卫生引用本文复制引用
李烽,朱振兴,李志欣,邵银进,朱惠芳.."补肾填精"中药复方改善脑瘫大鼠运动发育落后的机制研究[J].赣南医学院学报,2024,44(1):15-21,7.基金项目
江西省中医药管理局科技计划项目(2022B1057) （2022B1057）
江西省卫生健康委员会科技计划资助项目(202311918) （202311918）
