赣南医学院学报2024，Vol.44Issue(1)：22-26,5.DOI:10.3969/j.issn.1001-5779.2024.01.004
钙敏感受体在血吸虫病肝纤维化中的作用
The role of CaSR in liver fibrosis caused by schistosomiasis
摘要
Abstract
Objective:To investigate the role of calcium sensitive receptorsin schistosomiasis liver fibrosis.Methods:The BALB/c mice were used to establish a liver fibrosis model of schistosomiasis.The HE and Masson staining were used to observe pathological changes in mouse liver tissues.The Immunohistochemistry,RT-qPCR,and Western blot techniques were used to detect the expression of CaSR and fibrosis related indicators in the liver tissues.The ALT and AST assay kits were used to detect the content of liver tissue aminotransferase.The Sirius Red and VG staining were used to observe the effect of CaSR inhibitor(Calhex231)on liver fibrosis in mice.Results:The HE staining was found that the liver cells of the uninfected group mice were arranged neatly,while a large number of the eggs were found in the liver tissue of the infected group mice,with collagen fibrosis deposition around the eggs,forming granulomas of the eggs.The Masson staining results showed that the liver cells of the uninfected group mice were arranged neatly,and no obvious collagen deposition was observed in the liver tissues.While the liver tissue of the infected group mice was extensively damaged,with a large number of pseudolobules and a large amount of collagen fibrosis proliferation around pseudolobules.Compared with the uninfected group,the expressions of α-SMA,Collagen-Ⅰ,and CaSR in the liver tissue of the infected group mice were increased,and the contents of ALT and AST increased,and the differences were statistically significant(P＜0.05).The Sirius Red staining results showed that the liver tissue structure of the infected mice had been severely damaged,and a large amount of collagen fibrosis deposition had occurred;the CaSR inhibitor intervention group showed that damage to the liver tissue structure of the mice,but there were still some tissues with complete structures.The VG staining results showed that the liver tissue of the infected group mice had already shown a large amount of pink,while the liver tissue of the CaSR inhibitor intervention group mice showed partial pink,with some complete tissue structures.Conclusion:CaSR may play a role in schistosomiasis liver fibrosis.关键词
日本血吸虫/肝纤维化/钙敏感受体Key words
Schistosoma japonicum/Liver fibrosis/Calcium-sensitive receptor分类
基础医学引用本文复制引用
张文娟,曹镐禄,李珂云,冯嘉禾,林乐迎,熊俊,秦静茹,胡衡,李珍妮,贺喆..钙敏感受体在血吸虫病肝纤维化中的作用[J].赣南医学院学报,2024,44(1):22-26,5.基金项目
江西省教育厅科学技术研究项目(GJJ2201410) （GJJ2201410）
