摘要
Abstract
Objective:To evaluate the potential value of disulfide death-related LncRNA as prognostic indicators in patients with hepatocellular carcinoma(HCC).Methods:RNA-Seq and clinical information data of HCC were obtained from the TCGA database,and 10 bisulfide death-associated genes were obtained from related literature,and bisulfide death-associated LncRNA were obtained by co-expression analysis.Then,a prognostic model based on bisulfite death-associated LncRNA was constructed by using one-factor Cox regression,Lasso risk regression analysis,and multifactor Cox regression.The HCC samples were divided into high-risk and low-risk groups based on the median risk score values of the training set,and the model effects were assessed by K-M survival and ROC curves,principal component analysis(PCA),and C-index.In addition,GSEA enrichment analysis of differential genes between the two groups was performed,and differential analysis of immune function and immunotherapy effect were also analyzed.Results:Altogether 320 bisulfide death-associated LncRNA were screened from the TCGA-LIHC database,40 LncRNA associated with patients'prognosis were obtained by univariate Cox analysis(P＜0.05),and 6 prognostic models of bisulfide death-associated LncRNA were constructed by Lasso risk regression and multivariate Cox regression(ELFN1-AS1,AL049840.2,MKLN1-AS,CYTOR,AL161645.1,and AC069307.1),and the K-M survival and ROC curves,principal component analysis(PCA),and the C-index verified the good predictive ability of the models.GO and KEGG enrichment analyses were performed on the differential genes of the high-risk and low-risk groups.GO enrichment analysis showed that biological processes were mainly enriched in external encapsulating structure organisation,cellular components were mainly enriched in collagen-containing extracellular matrix,molecular functions are mainly enriched in extracellular matrix structural constituents.KEGG was mainly enriched in the PI3K/AKT signaling pathway.In addition,functional enrichment analysis of differentially expressed genes in the high-and low-risk groups revealed that the high-risk group was mainly enriched in the extracellular matrix receptor pathway,while the low-risk group was mainly enriched in the drug metabolism cytochrome p450 pathway.The GSEABase package was used to analyze the differences between the immune functions of the high-risk and low-risk groups and the results showed that the high-risk group had a higher prevalence of antigen-presenting cell co-stimulation(APC_CO_stimulation),chemokine receptor(CCR),major histocompatibility complex class Ⅰ presentation(MHC_class_Ⅰ),parainflammation and helper T-cell 2(Th2)expression were higher than those in the low-risk group,and the TIDE score was higher in the high-risk group than that in the low-risk group.Conclusion:A prognostic model of HCC with good predictive performance was constructed based on the TCGA database,which provides a new idea for further research on the role of disulfide death-associated LncRNA in hepatocellular carcinoma.关键词
肝细胞癌/癌症基因组图谱/双硫死亡/长非编码RNA/预后模型Key words
Hepatocellular carcinoma/The cancer genome atlas/Disulfide death/Long non-coding RNA/Prognostic model分类
医药卫生
