南方农业学报2023，Vol.54Issue(11)：3359-3368,10.DOI:10.3969/j.issn.2095-1191.2023.11.022
gga-miR-30d-5p表达分析及其对鸡脂肪沉积的调控作用
Expression analysis of gga-miR-30d-5p and its regulation of fat deposition in chickens
摘要
Abstract
[Objective]The purpose of the study was to analyze the effects of gga-miR-30d-5p on abdominal fat and in-tramuscular fat deposition of chicken,and to provide a theoretical basis for further elucidating the molecular mechanism of abdominal fat and intramuscular fat deposition in chickens.[Method]Real-time fluorescence quantitative PCR was used to detect the expression of gga-miR-30d-5p in different tissues of S3 line chickens and recessive white feather chick-ens at different developmental periods.The gga-miR-30d-5p mimics and inhibitor were transfected to observe the changes of fat deposition in adipocytes.[Result]The gga-miR-30d-5p was expressed in heart,liver,spleen,sebum,abdominal fat,chest muscle,kidney,ovary and leg tissues.In abdominal fat tissue,gga-miR-30d-5p expression at 0 week of age showed significant difference between breeds(P<0.05,the same below),and the expression at 0 week of age was signifi-cantly higher than that of other weeks of age.In liver tissues,there were significant breed differences in the expression of gga-miR-30d-5p at 0,14 and 16 weeks of age.In S3 line chickens,the expression of gga-miR-30d-5p increased gradually with the growth of developmental period,and the expression at 16 weeks of age was significantly higher than that of other weeks of age.In recessive white chickens,the expression was the highest at16 weeks of age significantly higher than that of 8 weeks.In leg muscle tissues,gga-miR-30d-5p expression at 2 weeks of age showed significant difference between breeds.In adipocyts,the expression of gga-miR-30d-5p was significantly higher than that in the proliferation period after 4 d after inducing differentiation.Lipid drop deposition was significantly increased in adipocytes after transfection with gga-miR-30d-5p mimics.Lipid drop deposition was significantly decreased in adipocytes after transfection with gga-miR-30d-5p inhibitor.The enrichment analysis results of GO and KEGG of target genes showed that SOCS3 and PGP may be the predicted target genes of gga-miR-30d-5p to regulate fat deposition.[Conclusion]The expression of gga-miR-30d-5p in chicken liver,abdominal fat and leg muscle tissues shows significant difference between breeds.Lipid drop deposition in adipocytes is significantly increased/decreased after transfection with gga-miR-30d-5p mimics/inhibitor.The gga-miR-30d-5p has the effects of promoting abdominal fat and intramuscular fat deposition in chickens.关键词
gga-miR-30d-5p/鸡/腹脂脂肪/肌内脂肪/脂肪沉积Key words
gga-miR-30d-5p/chicken/abdominal fat/intramuscular fat/fat deposition分类
农业科技引用本文复制引用
黄华云,李瑞瑞,张子晓,王钱保,黄正洋,李春苗,赵振华..gga-miR-30d-5p表达分析及其对鸡脂肪沉积的调控作用[J].南方农业学报,2023,54(11):3359-3368,10.基金项目
现代农业产业技术体系建设专项(CARS-41-Z05) （CARS-41-Z05）
江苏省种业振兴揭榜挂帅项目(JBGS(2021)109,JBGS(2021)029) （JBGS(2021）
国家家养动物种质库建设项目(资源库2023) China Agriculture Research System(CARS-41-Z05) （资源库2023）
Jiangsu Seeds Industry Revitalization Project(JBGS(2021)109),JBGS(2021)029) （JBGS(2021）
Construction Project of National Domestic Animal Germplasm Bank(Ziyuanku 2023) （Ziyuanku 2023）
