南方农业学报2023，Vol.54Issue(11)：3156-3164,9.DOI:10.3969/j.issn.2095-1191.2023.11.003
外源生长素影响百合地下茎生鳞茎发生的转录组分析
Transcriptome analysis of exogenous auxin effect on lily underground stem bulbils formation
摘要
Abstract
[Objective]To reveal the molecular mechanism of hormone regulation on the occurrence and development of lily underground stem bulbs,and to provide reference for further development of the molecular mechanism of auxin re-lated genes regulation of lily underground stem bulbs.[Method]The underground stems of lily were treated with exog-enous auxin indoleacetic acid(IAA)at concentrations of 0.2 and 2.0 mmol/L,and water treatment was used as the con-trol(CK).The quantity and weight of harvested underground stem bulbs were measured.Combined with transcriptome se-quencing analysis,the temporal and spatial expression patterns of various hormone-related genes under different auxin concentrations were revealed.Real-time fluorescence quantitative PCR was used to detect the expression level of key genes in lily lateral organogenesis.[Result]The total number of stem bulbs,number of stem bulbs per plant and average gram weight of stem bulbs of lily plants treated with 2.0 mmol/L concentration IAA were lower than those treated with 0.2 mmol/L concentration auxin and control group.The results of KEEG enrichment pointed to the plant hormone signal trans-duction pathway.The digital expression profiles of differential genes showed that almost all genes related to plant hor-mones were affected,including auxin,ethylene,ababolic acid,brassinolide,methyl jasmonate,cytokinin and gibberel-lin,among which methyl jasmonate had the most differential genes,followed by auxin.Real-time fluorescence quantita-tive PCR detection showed that the expression levels of WOX9,WOX11,KNOX2,CYCD3 and CYCD5 genes in 2.0 mmol/L treatment were significantly lower than those in CK(P<0.05,the same below).[Conclusion]High concentration of auxin IAA can effectively delay the formation and development of underground stem bulbs of lily.Exogenous auxin treatment not only affects the anabolism,transport and signal transduction of many plant hormones,but also inhibits the expression of key genes of lily lateral organogenesis,thus affecting the formation and development of underground stem bulbs.关键词
百合/生长素/茎生鳞茎/转录组测序/珠芽Key words
lily/auxin/stem bulbs/transcriptome sequencing/bulbil分类
农业科技引用本文复制引用
杨成龙,方少忠,黄永旺,陈佩振,林智敏..外源生长素影响百合地下茎生鳞茎发生的转录组分析[J].南方农业学报,2023,54(11):3156-3164,9.基金项目
福建省自然科学基金项目(2023J01366) Fujian Natural Science Foundation(2023J01366) （2023J01366）
