南方农业学报2023，Vol.54Issue(11)：3302-3313,12.DOI:10.3969/j.issn.2095-1191.2023.11.017
超声波辅助提取地涌金莲多酚及其抗氧化活性分析
Ultrasonic-assisted extraction of polyphenols from Musella lasiocarpa(Franch.)and antioxidant activity of the extracted polyphenols
摘要
Abstract
[Objective]The response surface method was used to optimize the process conditions of ultrasound-assisted extraction of polyphenols from Musella lasiocarpa(Franch.),and the antioxidant activities of M.lasiocarpa poly-phenol extract(MLPE)were further evaluated,to provide theoretical support for further development and utilization of M.lasiocarpa.[Method]Ultrasonic power(X1),extraction temperature(X2),extraction time(X3)and solid-liquid ratio(X4)were selected as experimental factors,and polyphenol yield(Y)was the response value,response surface methodol-ogy was conducted to optimize conditions of ultrasound-assisted extraction from M.lasiocarpa petal on the basis of single factor experiments.The antioxidant activity of the polyphenol extract was evaluated by the scavenging ability of 1,1-diphe-nyl-2-trinitrophenylhydrazine(DPPH)free radicals and 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diamiam-monium salt(ABTS)and total antioxidant capacity(FRAP).[Result]The effect of each factor on the extraction rate of polyphenol from the M.lasiocarpa was X2>X3>X1>X4.The effect of interaction between X1X2 and X1X3 on the polyphenol extraction rate were extremely significant(P<0.01),while the effect of interaction effect of X2X3 was signifi-cant(P<0.05).The optimized model equation was Y=100.73-4.87X1X2-5.03X1X3-4.09X2X3-11.13X12-9.06X22-12.05X32-10.46X42,and the optimal extraction process parameter for polyphenols from the M.lasiocarpa were ultrasonic power of 360 W,extraction temperature of 55℃,extraction time of 30 min,and liquid-solid ratio of 1∶20(g/mL).Under these op-timal conditions,the extraction amount of polyphenols from M.lasiocarpa was 10.31 mg/g,which was consistent with the theoretical value of 10.08 mg/g.Results from antioxidant assays showed that the half maximal inhibitory concentration(IC50)values of DPPH and ABTS radical scavenging activity were 20.03 and 39.68 μg/mL,respectively,with a FRAP values of 47.54 μg/mL,antioxidant ability were higher compared to quercetin.[Conclusion]This study indicated that MLPE extracted by ultrasonic assisted extraction has good antioxidant activity,which can be developed into a edible,safe and natural antioxygen.关键词
地涌金莲/多酚/超声波辅助提取/抗氧化活性Key words
Musella lasiocarpa(Franch.)/polyphenols/ultrasonic assisted extraction/antioxidant activities分类
园艺学与植物营养学引用本文复制引用
韩明冲,周旭红,李南,茹月蓉,镡思怡,王振兴..超声波辅助提取地涌金莲多酚及其抗氧化活性分析[J].南方农业学报,2023,54(11):3302-3313,12.基金项目
中央引导地方科技发展资金科技创新基地建设项目(202207AB110015) （202207AB110015）
云南省教育厅科学研究基金项目(2021Y271) （2021Y271）
云南省大学生创新创业训练计划项目(2020) （2020）
西南林业大学生物学质量工程项目(503190106) Central Guidance for Local Science and Technology Development Fund(202207AB110015) （503190106）
Scientific Research Project of Yunnan Education Department(2021Y271) （2021Y271）
Training Project of College Students'Innova-tion and Entrepreneurship in Yunnan(2020) （2020）
Biological Quality Engineering Project of Southwest Forestry University(503190106) （503190106）
