|国家科技期刊平台
首页|期刊导航|湖南农业大学学报(自然科学版)|醇化烟叶关键微生物类群与风格特色的相关性

醇化烟叶关键微生物类群与风格特色的相关性OA北大核心CSTPCD

Key microbial groups are closely related to the formation of aroma characteristics in aged tobacco leaves

中文摘要英文摘要

分别选取清香型(云南玉溪)、浓香型(湖南郴州)和中间香型(贵州遵义)醇化 1 年的 C2F 烟叶,测定烟叶的主要化学成分;利用相似度检验分析烟叶微生物群落差异物种,随机矩阵理论构建分子生态网络;运用Mantel分析烟叶主要化学成分与微生物群落的相关性,结果表明:清香型、中间香型和浓香型烟叶微生物群落结构存在显著差异,浓香型烟叶细菌及真菌群落 ɑ 多样性指数最高;烟叶显著富集的关键细菌类群为假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)和鞘氨醇单胞菌属(Sphingomonas);关键真菌类群为链格孢属(Alternaria)、根霉菌属(Rhizopus)和桑帕约氏酵母菌属(Sampaiozyma);以浓香型烟叶微生物分子生态网络规模最大,结构复杂且相对稳定.总糖与还原糖含量显著影响烟叶微生物群落的多样性与结构,清香型及中间香型烟叶内关键微生物类群均与总糖、还原糖含量呈显著正相关,而浓香型烟叶关键微生物类群均与总糖、还原糖含量呈显著负相关.

Clear-flavored,intermediate-flavored,and strongly flavored tobacco leaves respectively from Yuxi of Yunnan,Chenzhou of Hunan,and Zunyi of Guizhou,which had been mellowed for 1 year,were selected to test the main chemical composition of the tobacco leaves.The similarity test was used to analyze the differential species of the microbial community,the molecular ecological network was constructed based on the random matrix theory,and the correlation analysis was carried out by Mantel test.The results showed that there were significant differences in the structure of microbial communities among the three flavored tobacco leaves and the highest ɑ diversity index of bacterial and fungal communities were found in the strongly flavored tobacco leaves.Pseudomonas,Enterobacter,and Sphingomonas were the key bacterial groups and Alternaria,Rhizopus,and Sampaiozyma were the key fungi that were enriched in the three flavored tobacco leaves.The microbial ecological network of strong flavor tobacco leaves was the largest among the three flavored tobacco leaves,and the network was complex and relatively stable.Total sugar and reducing sugar content significantly affected the diversity and structure of the microbial community of tobacco leaves.The key microbial taxa of clear and intermediate flavor tobaccos were significantly positively correlated with the total sugar and reducing sugar content,whereas the key microbial taxa of strong flavor tobaccos were significantly negatively correlated with the total sugar and reducing sugar content.

刘晓敏;雷佳;黎娟;黄晓杰;蔡昊城;何沛;朱志扬;朱益;彭光爵;秦天;马婷婷

云南省烟草化学重点实验室,云南 昆明 650231湖南农业大学农学院,湖南 长沙 410128云南省烟草公司临沧市公司双江分公司,云南 临沧 677300常德市烟草公司,湖南 常德 415000

农业科学

醇化烟叶烟叶香型化学成分微生物多样性关键类群相关性

aged tobacco leavestobacco aroma typechemical compositionmicrobial diversitykey taxacorrelation

《湖南农业大学学报(自然科学版)》 2024 (001)

23-30 / 8

云南中烟工业有限责任公司科技项目(2021539200340245)

10.13331/j.cnki.jhau.2024.01.004

评论