湖南农业大学学报（自然科学版）2024，Vol.50Issue(1)：43-51,9.DOI:10.13331/j.cnki.jhau.2024.01.007
OsmiR530正调控水稻的耐旱和耐盐性
OsmiR530 positively regulates the drought and salt tolerance of rice
摘要
Abstract
Drought and salt tolerance related functions were identified on the transgenic OsmiR530-OE rice mediated by Agrobacterium tumefacience and Nipponbare.The results showed that two mature sequences of OsmiR530,OsmiR530-5P and OsmiR530-3P existed in the plant,and the expression of OsmiR530 was significantly increased in the OsmiR530-OE transgenic plants.The expression levels of predicted target genes OsTAP46,OsC3HC4,OsPWI and OsE1 of OsmiR530-5p were down-regulated in OsmiR530-OE transgenic plants.Under drought and salt stress,OsmiR530 gene could increase germination rate,root length,seedling height and seedling tolerance to drought and salt stress.SOD,POD and CAT activities of drought-treated plants were detected,and it was found that SOD,POD and CAT activities of OsmiR530-OE transgenic rice increased significantly.The statistical analysis of agronomic traits of rice plants at maturity showed that the leaf surface area,thousand grain weight and seed setting rate of sword leaf of rice plants overexpressing OsmiR530 were significantly reduced.Most likely,OsmiR530 could regulate the tolerance of rice to salt and drought stress.关键词
水稻/miR530/耐旱性/耐盐性Key words
rice/miR530/drought tolerance/salt tolerance分类
农业科技引用本文复制引用
吕思晓,肖敏敏,陈信波..OsmiR530正调控水稻的耐旱和耐盐性[J].湖南农业大学学报（自然科学版）,2024,50(1):43-51,9.基金项目
湖南农业大学"双一流"学科建设项目(SYL2019019) （SYL2019019）
