OsmiR530正调控水稻的耐旱和耐盐性OA北大核心CSTPCD
OsmiR530 positively regulates the drought and salt tolerance of rice
以粳稻品种日本晴(CK)和农杆菌介导转化的OsmiR530-OE转基因水稻为材料进行耐旱和耐盐性相关功能的鉴定.结果表明:OsmiR530有 2种成熟的序列形式OsmiR530-5p和OsmiR530-3p,其表达量在OsmiR530-OE转基因植株均有明显增加;靶基因表达量预测分析发现,OsmiR530-5p 的靶基因 OsTAP46、OsC3HC4、OsPWI和OsE1等在OsmiR530-OE转基因植株中的表达都有一定程度的下调;在干旱、盐胁迫条件下,OsmiR530能提高种子的发芽率、根长和苗高以及萌芽期幼苗对干旱胁迫和盐胁迫的耐受性;检测干旱处理植株的 SOD、POD和CAT活性,发现OsmiR530-OE转基因水稻的SOD、POD和CAT活性增长显著;对成熟期水稻植株的农艺性状进行统计分析,发现过表达 OsmiR530 水稻植株的剑叶的叶表面积、千粒质量以及结实率显著降低.推测OsmiR530可以调控水稻对盐胁迫、干旱胁迫的耐受性.
Drought and salt tolerance related functions were identified on the transgenic OsmiR530-OE rice mediated by Agrobacterium tumefacience and Nipponbare.The results showed that two mature sequences of OsmiR530,OsmiR530-5P and OsmiR530-3P existed in the plant,and the expression of OsmiR530 was significantly increased in the OsmiR530-OE transgenic plants.The expression levels of predicted target genes OsTAP46,OsC3HC4,OsPWI and OsE1 of OsmiR530-5p were down-regulated in OsmiR530-OE transgenic plants.Under drought and salt stress,OsmiR530 gene could increase germination rate,root length,seedling height and seedling tolerance to drought and salt stress.SOD,POD and CAT activities of drought-treated plants were detected,and it was found that SOD,POD and CAT activities of OsmiR530-OE transgenic rice increased significantly.The statistical analysis of agronomic traits of rice plants at maturity showed that the leaf surface area,thousand grain weight and seed setting rate of sword leaf of rice plants overexpressing OsmiR530 were significantly reduced.Most likely,OsmiR530 could regulate the tolerance of rice to salt and drought stress.
吕思晓;肖敏敏;陈信波
湖南农业大学生物科学技术学院,湖南 长沙 410128
农业科学
水稻miR530耐旱性耐盐性
ricemiR530drought tolerancesalt tolerance
《湖南农业大学学报（自然科学版）》 2024 (001)
43-51 / 9
湖南农业大学"双一流"学科建设项目(SYL2019019)
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