湖南农业大学学报（自然科学版）2024，Vol.50Issue(1)：58-62,5.DOI:10.13331/j.cnki.jhau.2024.01.009
黄连生物碱对TGEV诱导的ST细胞炎症反应的调节作用
The inflammatory response regulating role of alkaloids of Coptis chinensis in swine testis cells induced by TGEV
摘要
Abstract
The swine testicular(ST)cells infected with transmissible gastroenteritis virus(TGEV)were treated with alkaloids of Coptis chinensis(ACC)with mass concentrations of 100,50 and 25 μg/mL respectively.MTT assay was used to detect the cell viability,the griess method and colorimetric method were used to detect the release of NO,TNOS and iNOS,and Real-time PCR method was used to detect expression changes of inflammatory genes such as TNF-α,IL-1β,IL-6 and IL-10.The results indicated that ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly increase the survival rate of ST cells,and 25 μg/mL ACC could significantly increase the survival rate of ST cells.The content of NO increased sharply after TGEV infection,and the ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly decrease the activities of TNOS and iNOS,and 25 μg/mL ACC could significantly decrease the activities of enzymes TNOS and iNOS.Meanwhile,ACC could extremely significantly down-regulate the expression levels of TNF-α,IL-1β,IL-6 and IL-10 mRNA.We can conclude that the alkaloids of Coptis chinensis could reduce the inflammatory response induced by TGEV and alleviate the damage of virus to cells.关键词
黄连生物碱/猪传染性胃肠炎病毒/猪睾丸细胞/炎症相关基因Key words
alkaloids of Coptis chinensis/transmissible gastroenteritis virus/swine testis cells/inflammation-related genes分类
农业科技引用本文复制引用
唐红梅,朱买勋,邓小龙,徐登峰..黄连生物碱对TGEV诱导的ST细胞炎症反应的调节作用[J].湖南农业大学学报（自然科学版）,2024,50(1):58-62,5.基金项目
重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0757) （cstc2020jcyj-msxmX0757）
国家重点研发计划项目(2022YFD1601403) （2022YFD1601403）
国家生猪技术创新中心先导项目(NCTIP-XD/B12) （NCTIP-XD/B12）
