黄连生物碱对TGEV诱导的ST细胞炎症反应的调节作用OA北大核心CSTPCD

The swine testicular(ST)cells infected with transmissible gastroenteritis virus(TGEV)were treated with alkaloids of Coptis chinensis(ACC)with mass concentrations of 100,50 and 25 μg/mL respectively.MTT assay was used to detect the cell viability,the griess method and colorimetric method were used to detect the release of NO,TNOS and iNOS,and Real-time PCR method was used to detect expression changes of inflammatory genes such as TNF-α,IL-1β,IL-6 and IL-10.The results indicated that ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly increase the survival rate of ST cells,and 25 μg/mL ACC could significantly increase the survival rate of ST cells.The content of NO increased sharply after TGEV infection,and the ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly decrease the activities of TNOS and iNOS,and 25 μg/mL ACC could significantly decrease the activities of enzymes TNOS and iNOS.Meanwhile,ACC could extremely significantly down-regulate the expression levels of TNF-α,IL-1β,IL-6 and IL-10 mRNA.We can conclude that the alkaloids of Coptis chinensis could reduce the inflammatory response induced by TGEV and alleviate the damage of virus to cells.