解放军医学杂志2024，Vol.49Issue(2)：214-219,6.DOI:10.11855/j.issn.0577-7402.1838.2023.0824
甘草酸对高糖诱导的肾小球系膜细胞炎性因子及纤维化因子的影响
Effect of glycyrrhizin on inflammatory factors and fibrotic factors in high glucose-induced glomerular mesangial cells
摘要
Abstract
Objective To investigate the effect of glycyrrhizic acid(GA)on the inflammatory and fibrotic factors in high glucose-induced glomerular mesangial cells(SV40 MES13).Methods Cultured mouse SV40 MES13 were divided into normal group(NG,5.6 mmol/L glucose),high glucose group(30 mmol/L glucose)and HG+GA group(30 mmol/L glucose+200 μmol/L GA).The expression levels of inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),IL-6,IL-8 and α-smooth muscle actin(α-SMA)in different groups were detected by Western blotting.The fluorescence intensity of IL-1β,TNF-α and α-SMA in different groups were detected by immunofluorescence.The levels of IL-1β,TNF-α,IL-6 and IL-8 in the culture supernatant of different populations were detected by enzyme-linked immunosorbent assay(ELISA).Results The protein expressions of IL-1β,TNF-α,IL-6,IL-8 and α-SMA in HG group were significantly higher than those in NG group(P<0.01);Compared with HG group,the protein expression levels of IL-1β,TNF-α,IL-6,IL-8 and α-SMA decreased significantly in HG+GA group(P<0.05).The fluorescence intensity of inflammatory cytokines IL-1β,TNF-α and α-SMA increased in HG group than those in NG group(P<0.05);While compared with the HG group,the fluorescence intensity of IL-1β,TNF-α and α-SMA in HG+GA group decreased markedly(P<0.05).The experimental results of ELISA showed that compared with NG group,the levels of IL-1β,IL-6,TNF-α and IL-8 in cell supernatent increased in HG group(P<0.01);while the levels of IL-1β,TNF-α,IL-6,IL-8 in HG+GA group significantly lower than those in HG group(P<0.05).Conclusion Glycyrrhizic acid has certain inhibitory effect on high glucose-induced inflammatory factors and fibrotic factors in glomerular mesangial cells,which may play an important role in prevention of diabetic nephropathy.关键词
糖尿病肾病/高糖/甘草酸/肾小球系膜细胞/炎性因子/纤维化Key words
diabetic nephropathy/high glucose/glycyrrhizic acid/glomerular mesangial cells/inflammatory factor/fibrosis分类
医药卫生引用本文复制引用
李媛,王珍,曹雪,侯绍章..甘草酸对高糖诱导的肾小球系膜细胞炎性因子及纤维化因子的影响[J].解放军医学杂志,2024,49(2):214-219,6.基金项目
This work was supported by the Ningxia Natural Science Foundation Project(2021AAC03125) 宁夏自然科学基金(2021AAC03125) （2021AAC03125）
