摘要
Abstract
To investigate the effects and mechanism of anlotinib intervention on glycolysis,proliferation,colony formation and migration of human lung cancer NCI-H226 cells,to provide reference for in vitro test of lung cancer.Human lung cancer NCI-H226 cells were divided into control group which is without intervention,experimental group involving 10,20,40 μmol/L anlotinib,and positive control group involving 20 μg/mL cisplatin.The cell counting kit-8(CCK-8),5-acetylene-2′ deoxyura-cil riboside(EdU)staining,plate clone formation,lactic acid detection kit,glucose detection kit,Transwell assay,RT-qPCR and Western blotting(WB)assays were used to detect proliferation viability,proliferation,colony-formation ability,lactic acid con-tent,glucose consumption level,cell migration number and expression level of related factor levels.The proliferation activity,proliferation rate,clone formation rate,lactate content,glucose consumption level,N-cadherin,vimentin and fibronectin(FN)of NCI-H226 cells in control group,experimental group and positive drug group.There were significant differences in mRNA and protein expression levels between groups(P<0.05).The cell proliferation activity of 20 and 40 μmol/L anlotinib and positive drug groups was higher than that of control group(P<0.05).The cell proliferation rate,clone formation rate,lactate content,glucose consumption level and mRNA and protein expression levels of N-cadherin,vimentin and FN in the experimental group and the positive drug group were higher than those in the control group(P<0.05).Anlotinib can significantly inhibit glycolysis,proliferation,colony formation and migration of human lung cancer NCI-H226 cells.关键词
安罗替尼/肺癌/糖酵解/癌细胞增殖/克隆形成Key words
anlotinib/lung cancer/glycolysis/proliferation of cancer cells/colony formation分类
医药卫生
