激光生物学报2024，Vol.33Issue(1)：90-96,7.DOI:10.3969/j.issn.1007-7146.2024.01.011
隐丹参酮对铜绿假单胞菌的生长及生物膜形成的抑制作用
Inhibitory Effect of Cryptotanshinone on Biofilm Formation of Pseudomonas aeruginosa
摘要
Abstract
To investigate the effects of cryptotanshinone on the growth and biofilm formation of Pseudomonas aeruginosa and its mechanism,the minimum inhibitory concentration(MIC)of cryptotanshinone on P.aeruginosa was determined by mi-crodilution,and the effect of cryptotanshinone on P.aeruginosa biofilm formation was observed by crystal violet staining,inverted microscope and laser scanning confocal microscope,and the effect of cryptotanshinone on P.aeruginosa biofilm for-mation was measured by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).The effect of cryptotanshi-none on the expression of genes related to P.aeruginosa biofilm formation was detected by RT-qPCR.The MIC of cryptotan-shinone on P.aeruginosa was 800.0 μg/mL;after crystal violet staining,the best inhibition of biofilm was observed at the mass concentration of cryptotanshinone of 800.0 μg/mL in a dose-dependent manner(P<0.01).The formation and distribution of biofilm by cryptotanshinone were observed by confocal laser microscopy,and the inhibition effect was significantly enhanced at 200.0 μg/mL.In the range of 200.0～800.0 μg/mL mass concentration,cryptotanshinone at 800.0 μg/mL mass concentra-tion inhibited the expression of LasI,LasR,RhlI and RhlR genes(P<0.01).The mechanism of cryptotanshinone inhibition of P.aeruginosa biofilm formation may be through the down-regulation of LasI and other genes expression.The present study suggests that cryptotanshinone can be used as a group-sensing inhibitor to intervene in the drug resistance of P.aeruginosa,which can provide a reference for the treatment of multi-drug-resistant P.aeruginosa(MDR-PA)in the clinic.关键词
铜绿假单胞菌/生物膜/隐丹参酮/群感系统/耐药性Key words
Pseudomonas aeruginosa/biofilm/cryptotanshinone/quorum sensing/drug resistance分类
医药卫生引用本文复制引用
马书恒,缪林坪,陈荻秋,韩丽,张富凯,贺气志..隐丹参酮对铜绿假单胞菌的生长及生物膜形成的抑制作用[J].激光生物学报,2024,33(1):90-96,7.基金项目
国家自然科学基金青年项目(82100040) （82100040）
湖南省教育厅科学研究项目(湘教通[2022]323号-22B0899) （湘教通[2022]323号-22B0899）
湖南省大学生创新创业训练项目(湘教通[2021]197号-3905) （湘教通[2021]197号-3905）
湖南省大学生创新创业训练计划重点支持领域项目(湘教通[2023]237号-38) （湘教通[2023]237号-38）
长沙医学院大学生创新创业训练项目(长医教[2023]51号-108). （长医教[2023]51号-108）
