基础医学与临床2024，Vol.44Issue(3)：333-338,6.DOI:10.16352/j.issn.1001-6325.2024.03.0333
桃叶珊瑚苷抑制人肝癌细胞系HepG2增殖
Aucubin inhibits the proliferation of human hepatocellular carcinoma cells line HepG2
摘要
Abstract
Objective To investigate the effects of aucubin(AU)on the proliferation,apoptosis,and cell cycle of human liver cancer cells line HepG2 and its mechanism of action.Methods HepG2 cells were cultured in vitro,CCK-8 method was applied to screen the optimal dosage concentration of AU.HepG2 cells were randomly grouped into a control group,an AU 12.5 mg/L group(AU L group),an AU 62.5 mg/L group(AU H group),and an AU H+Akt pathway agonist(SC79)group(AU H+SC79 group).The cell proliferation was observed in each group;5-Ethynyl-2′-deoxyuridine(EDU)method was applied to detect cell proliferation;Flow cytometry was applied to detect cell apoptosis and cell cycle;Western blot was applied to detect the expression levels of phosphorylated pro-tein kinase B(p-Akt),Akt,p-MDM2,MDM2,p-p53,and p53 proteins.Results AU concentrations of 12.5 and 62.5 mg/L were selected for subsequent experiments.Compared with 0 mg/L AU,the proliferation of 12.5 and 62.5 mg/L AU cells was obviously reduced(P＜0.05);Compared with the control group,the number of suspended and exfoliated cells in the AU L and AU H groups gradually increased.Cells shrunk and became round.The propor-tion of G0/G1 phase cells,the proportion of EDU positive staining cells increased and the expression level of p-Akt/Akt and p-MDM2/MDM2 proteins decreased.The proportions of S and G2/M phase cells,the rate of cell apoptosis,and the expression level of p-p53/p53 protein all increased(P＜0.05).Compared with the AU H group,the above changes in the AU H+SC79 group were recovered(P＜0.05).After AU treatment,the tumor vol-ume and weight of transplanted nude mice decreased.Conclusions AU may inhibit the proliferation of liver cancer cells,induce cell cycle arrest and apoptosis by regulating the Akt/MDM2/p53 signaling pathway.关键词
桃叶珊瑚苷/肝癌细胞/增殖/凋亡/细胞周期Key words
aucubin/liver cancer cell/proliferation/apoptosis/cell cycle分类
医药卫生引用本文复制引用
安琪,齐光照,韩超..桃叶珊瑚苷抑制人肝癌细胞系HepG2增殖[J].基础医学与临床,2024,44(3):333-338,6.基金项目
河南省医学科技攻关计划项目(LHGJ20190270) （LHGJ20190270）
