解放军医学杂志2024，Vol.49Issue(2)：165-170,6.DOI:10.11855/j.issn.0577-7402.0075.2023.0427
成人接种2剂新冠灭活疫苗12个月后T细胞免疫应答特点
Characteristics of T cell immune responses in adults inoculated with 2 doses of SARS-CoV-2 inactivated vaccine for 12 months
摘要
Abstract
Objective To evaluate the characteristics of different antigen-specific T cell immune responses to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)after inoculation with 2 doses of SARS-CoV-2 inactivated vaccine for 12 months.Methods Fifteen healthy adults were enrolled in this study and blood samples collected at 12 months after receiving two doses of SARS-CoV-2 inactivated vaccine.The level and phenotypic characteristics of SARS-CoV-2 antigen-specific T lymphocytes were detected by activation-induced markers(AIM)based on polychromatic flow cytometry.Results After 12 months of inoculation with 2 doses of SARS-CoV-2 inactivated vaccine,more than 90%of adults had detectable Spike and Non-spike antigen-specific CD4+ T cells immune responses(Spike:14/15,P=0.0001;Non-spike:15/15,P<0.0001).80%of adults had detectable Spike and Non-spike antigen-specific CD8+ T cells immune responses(Spike:12/15,P=0.0463;Non-spike:12/15,P=0.0806).Antigen-specific CD4+ T cells induced by SARS-CoV-2 inactivated vaccination after 12 months were composed of predominantly central memory(CM)and effector memory 1(EM1)cells.On the other hand,in terms of helper subsets,antigen-specific CD4+ T cells mainly showed T helper 1/17(Th1/17)and T helper 2(Th2)phenotypes.Conclusions SARS-CoV-2 inactivated vaccination generates durable and extensive antigen-specific CD4+ T cell memory responses,which may be the key factor for the low proportion of severe coronavirus disease 2019(COVID-19)infection in China.关键词
新型冠状病毒/灭活疫苗/T细胞免疫应答Key words
severe acute respiratory syndrome coronavirus 2/inactivated vaccine/T cell immune response分类
医药卫生引用本文复制引用
王静,王福生,李亚群,汪海燕,宋曜如,李静,王文鑫,万林钰,周春保,范兴..成人接种2剂新冠灭活疫苗12个月后T细胞免疫应答特点[J].解放军医学杂志,2024,49(2):165-170,6.基金项目
This work was supported by the Guangzhou Laboratory Emergency Response Project(EKPG21-30-4) 广州实验室应急攻关项目(EKPG21-30-4) （EKPG21-30-4）
