昆明医科大学学报2024，Vol.45Issue(2)：32-38,7.DOI:10.12259/j.issn.2095-610X.S20240205
慢性肾衰竭大鼠OAT1的表达对骨代谢的影响
Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure
摘要
Abstract
Objective To investigate the changes of OAT expression in bone cells in chronic renal failure(CRF)and involved mechanism,and to explore the effect of OAT expression on bone metabolism.Methods Ra-ndomly divide the rats into a control group(n=6)and a model group(n=6).The model group established a rat chronic renal failure model using"single nephrectomy+adenine gavage"method,and the red blood cell(RBC)and hemoglobin(HGB)of the rat body were measured using a blood routine analyzer;Measure indicators such as creatinine(Cr),urea nitrogen(BUN),uric acid(UA),blood calcium(Ca2+),and blood phosphorus(P3+)using a fully automated biochemical analyzer;Pathological examination of rat kidneys;X-ray examination of rat tibia;Immunohistochemical examination of bone tissue OAT1 level.Results The bone density of the model group rats is lower than that of the control group;The calcium and phosphorus metabolism of rats in the model group was in metabolic disorder,and the OAT1 value of bone tissue binding was much lower than that of the control group(P= 0.0018),which was statistically significant.(P=0.0018)Conclusion Chronic renal failure affected the binding ability of OAT1 in bone tissue,leading to the metabolic disorder for calcium absorption and phosphorus metabolism,thus aggravating renal osteodystrophy(P<0.05).关键词
有机阴离子转运蛋白/慢性肾功能衰竭/骨营养不良Key words
Organic anion transporter/Chronic renal failure/Osteodystrophy分类
医药卫生引用本文复制引用
沈文娟,梁纹鑫,李清,徐宏芳,张伟琴,李海欣..慢性肾衰竭大鼠OAT1的表达对骨代谢的影响[J].昆明医科大学学报,2024,45(2):32-38,7.基金项目
云南省教育厅科学研究基金资助项目(2021J0880,2024J1236) （2021J0880,2024J1236）
