昆明医科大学学报2024，Vol.45Issue(2)：39-48,10.DOI:10.12259/j.issn.2095-610X.S20240206
MSC-exo一种新型细胞递送工具转运靶向基因调控胰腺癌增殖效应分析
Effect of MSC-exo,a New Cell Delivery Tool,on Gene Delivery and Proliferation of Pancreatic Cancer
摘要
Abstract
Objective To observe the effect of a new cell delivery tool(MSC exo)on the proliferation of pancreatic cancer by transferring targeted genes.Methods Transmission Electron Microscope(TEM)and Nanoparticle Tracking Analysis(NTA)were used to identify human mesenchymal stem cell exosomes(MSC-exo)and transport miR-450a-5p into CFPAC-1,to explore the effect of miR-450a-5p targeting BZW2 on inhibiting the proliferation of pancreatic cancer cells.Results The expression of miR-450a-5p was low in pancreatic cancer tissue(P<0.05),and the expression of CD63 and TSG101 of MSC-exo-miR-450a-5p in CFPAC-1 cells was higher than that of MSC-exo by Western blot(P<0.05).CCK-8 and EdU results showed that MSC-exo-miR-450a-5p significantly inhibited the proliferation of CFPAC-1 cells(P<0.05).Cell scratch and Transwell experiments showed that MSC-exo-miR-450a-5p can inhibit the migration and invasion of CFPAC-1 cells(P<0.05).Through dual luciferase assay,it was confirmed that miR-450a-5p targets BZW2,and RT-qPCR and Western blotting showed a negative correlation(P<0.05)between miR-450a-5p and BZW2 expression.Overexpression of BZW2,CCK-8,EdU,cell scratch,and Transwell experiments confirmed that pc-BZW2 reversed the anti-cancer function of MSC-exo-miR-450a-5p on CFPAC-1.Western blot detected PCNA,Ki-67,MMP2,MMP9,and the results were consistent with the above experiments(P<0.05).Conclusion hMSC exo is a new delivery system,targeting BZW2 to transport miR-450a-5p to inhibit the biological malignancy of pancreatic cancer cells,which provides an important clue for the research of targeted treatment of pancreatic cancer.关键词
胰腺癌/间充质干细胞/外泌体/miR-450a-5p/BZW2Key words
Pancreatic adenocarcinoma/Mesenchymal stem cell/Exosomes/miR-450a-5p/BZW2分类
医药卫生引用本文复制引用
朱磊,李瑞雪,鲍长磊,黄晨宸,梁书鑫,赵振林,朱洪..MSC-exo一种新型细胞递送工具转运靶向基因调控胰腺癌增殖效应分析[J].昆明医科大学学报,2024,45(2):39-48,10.基金项目
深圳市科技创新基础研究基金资助项目(JCYJ20190807103605679) （JCYJ20190807103605679）
