昆明医科大学学报2024，Vol.45Issue(2)：77-84,8.DOI:10.12259/j.issn.2095-610X.S20240210
肠道菌群代谢物TMAO与非酒精性脂肪性肝病的关系
The Association of Intestinal Flora Metabolite TMAO with Non-alcoholic Fatty Liver Disease
摘要
Abstract
Objective To explore the role of TMAO from gut microbiota in non-alcoholic fatty liver disease(NAFLD),we detected the serum level of TMAO and its precursor metabolites in NAFLD,as well as the expression level of Eubacterium rectum,Bacteroidetes multiforme,Lactobacillus and bifidobacterium in the intestinal flora.Methods We collected 118 subjects and divided into NAFLD group(86 cases)and healthy control group(32 cases)randomly.We also detected the serum level of TMAO and its precursor metabolites in subjects by high performance liquid chromatography tandem mass spectrometry detection(LC-MS),and the expression of target bacterial DNA was detected by qRT-PCR.Results Serum TMAO,TMA and choline levels were significantly increased in NAFLD(P<0.05),and liver fat content was positively correlated with TMAO(P<0.05).The expression level of Lactobacillus and Eubacterium rectum in NAFLD group were increased(P<0.05);the expression level of Bifidobacterium and Bacteroides multiform were decreased(P<0.05).The serum TMAO level was positively correlated with Eubacterium rectum(r=0.280,P<0.05),and negatively correlated with Bifidobacterium(r=-0.332,P<0.05).Conclusion The level of TMAO in serum shows a positive correlation with NAFLD.The structure of intestinal flora in individuals with NAFLD is altered and linked to TMAO.This suggests that the intestinal flora may have a significant impact on the development of NAFLD through TMAO.关键词
非酒精性脂肪性肝病/氧化三甲胺/三甲胺/肠道菌群Key words
Non-alcoholic fatty liver disease/TMAO/TMA/Intestinal flora分类
医药卫生引用本文复制引用
李媛媛,宋亚贤,徐玉善,曾晓甫,袁惠,徐兆,江艳..肠道菌群代谢物TMAO与非酒精性脂肪性肝病的关系[J].昆明医科大学学报,2024,45(2):77-84,8.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202001AY070001-195) （202001AY070001-195）
云南省代谢性疾病临床医学研究中心资助项目(202102AA100056) （202102AA100056）
云南省内分泌代谢性疾病临床医学中心资助项目(YWLCYXZXXYS20221005) （YWLCYXZXXYS20221005）
