Babao Dan Inhibits Gastric Cancer Progression in vivo Through Multiple Signaling PathwaysOA北大核心CSTPCD
Babao Dan Inhibits Gastric Cancer Progression in vivo Through Multiple Signaling Pathways
Objective:The aim of this study was to explore the effects of Babao dan(BBD),a traditional Chinese medicine,on gastric cancer(GC)progression in vivo.Materials and Methods:A subcutaneous xenograft mouse model of GC was established using MGC80-3 cells.The terminal deoxynucleotidyl transferase-mediated dUTP:2'-deoxyuridine 5'-triphosphate-biotin nick-end labeling method was adopted to detect cell apoptosis in vivo.The expression levels of proteins associated with proliferation,apoptosis,and angiogenesis were measured by immunohistochemical staining or western blotting(WB).The activation and protein levels of p-c-Jun N-terminal kinase(JNK),p-p38,p-extracellular-regulated kinase 1/2,p-nuclear factor-κB(NF-κB),and p-STAT3 were examined by Bio-plex and WB.Results:BBD significantly inhibited tumor growth in GC mouse models with no adverse effect on body weight or organ function.It was also found that BBD significantly suppressed the proliferation of GC tumor cells,induced the apoptosis of tumor cells,and inhibited angiogenesis through inactivating with mitogen-activated protein kinase,NF-κB,and STAT3 pathways.Conclusions:BBD exerts suppressive effects on GC tumor growth by regulating multiple pathways in vivo,which may provide a novel treatment option for GC therapy.
Hai-Xia Shang;Yi Fang;Bin Guan;Jian-Hua Guan;Jun Peng;Jin-Yan Zhao;Jiu-Mao Lin
Institute of Oncology of Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,ChinaInstitute of Oncology of Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China||Key Laboratory of Integrative Medicine of Fujian Province University,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China||Fujian Key Laboratory of Integrative Medicine on Geriatric,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China||Innovation and Transformation Center,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,ChinaXiamen Traditional Chinese Medicine Co.,Ltd.,Xiamen,Fujian,ChinaInstitute of Oncology of Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China||Key Laboratory of Integrative Medicine of Fujian Province University,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China||Fujian Key Laboratory of Integrative Medicine on Geriatric,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China
Babao dangastric cancermultiple signaling pathwaysproliferationtraditional Chinese medicine
《世界中医药杂志（英文版）》 2024 (001)
14-21 / 8
This study was financially supported by the Natural Science Foundation of Fujian Province,China(No.2019J01355).
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