日用化学工业（中英文）2024，Vol.54Issue(3)：282-289,8.DOI:10.3969/j.issn.2097-2806.2024.03.006
中药复方MHC-20的抗化脓性链球菌功效与作用机制探究
Efficacies and mechanisms of a formula of Chinese medicinal plants(MHC-20)against Streptococcus pyogenes
摘要
Abstract
The antimicrobial properties and mechanisms of a formula of Chinese medicinal plants(MHC-20)against Streptococcus pyogenes were studied.The MIC of MHC-20 against S.pyogenes was 31.25 μg/m L or 0.31%as measured by broth micro-or macro-dilution.MHC-20 was also found to have strong killing effects on Staphylococcus aureus,Escherichia coli and Candida albicans.The results of instantaneous bactericidal activity showed that MHC-20 was able to kill S.pyogenes completely in 5 min.It could also significantly reduce the cell surface hydrophobicity and inhibit the formation of biofilm.Cell membrane permeability test showed that MHC-20 could induce the increase of relative conductivity and alkaline phosphatase,as well as the protein leakage,in a concentration-dependent manner.The results of scanning electron microscopy further confirmed that MHC-20 had a strong destructive effect on cell morphology.Finally,a mouse model of cutaneous infection with S.pyogenes was established.It was shown that MHC-20 could promote wound healing and significantly inhibit the loading concentration of S.pyogenes(P＜0.01);5%MHC-20 and 20%MHC-20 significantly decreased the expression of IL-6 in serum and tissue of the mice(P＜0.001).20%MHC-20 significantly decreased the expression of leukocytes(P＜0.01).In conclusion,MHC-20 had the potential to be developed into a broad-spectrum antibiotic and an effective drug for cutaneous infection with S.pyogenes.关键词
化脓性链球菌/MHC-20/中药材/机制/皮肤感染Key words
Streptococcus pyogenes/MHC-20/Chinese medicinal plant/mechanism/skin infection分类
化学化工引用本文复制引用
胡翠翠,周代红,陈欣菀,钟佳伶,茆灿泉..中药复方MHC-20的抗化脓性链球菌功效与作用机制探究[J].日用化学工业（中英文）,2024,54(3):282-289,8.基金项目
四川省科技计划重点研发项目(2022YFS0437) （2022YFS0437）
