日用化学工业（中英文）2024，Vol.54Issue(3)：353-359,7.DOI:10.3969/j.issn.2097-2806.2024.03.015
高效液相色谱法测定化妆品中14种α-羟基酸和羟基酸酯
Determination of 14 kinds of α-hydroxy acids and hydroxy esters in cosmetics
摘要
Abstract
The study aimed to establish a method for simultaneous determination of 14 kinds of α-hydroxy acids and their esters in cosmetics by high performance liquid chromatography(HPLC),and the results were confirmed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).HPLC was used for gradient elution with 0.05 mol/L diammonium hydrogen phosphate-methanol as the mobile phase.The analytes were separated on a Waters T3-C18(4.6×250 mm,5µm)column and detected at the wavelengths of 214 nm.Quantification analysis was performed by the external standard method.HPLC-MS/MS was used for gradient elution with 0.1%formic acid-acetonitrile containing 0.1%formic acid as the mobile phase,and the analytes were separated on a Waters T3-C18(2.1×100 mm,1.8µm)column.Qualitative analysis was performed by electronic spray ionization under a positive ion and negative ion mode with multiple reaction monitoring(MRM).The linearity of the 14 components in the concentration range is good,with the correlation coefficient r≥0.999.The average spiked recoveries of 14 compounds in 4 cosmetic matrices are all in the range of 85%-115%,and the relative standard deviations are all less than 5%.The limits of detections range from 1 μg/g to 150 μg/g.The limits of quantitation range from 3 μg/g to 450 μg/g.Seven batches of samples containing α-hydroxy acid and its ester were confirmed by HPLC-MS/MS and the results are positive.The results indicate that the method is simple,sensitive and accurate,and is suitable for the determination of 14 kinds of α-hydroxy acids and their esters in cosmetics.关键词
化妆品/α-羟基酸/乳酸甲酯/乳酸乙酯/乳酸丁酯/高效液相色谱法Key words
cosmetics/α-hydroxy acids/methyl lactate/ethyl lactate/butyl lactate/high performance liquid chromatography(HPLC)分类
化学化工引用本文复制引用
张丽媛,颜琳琦,程巧鸳,戚绿叶,王容,黄柳倩..高效液相色谱法测定化妆品中14种α-羟基酸和羟基酸酯[J].日用化学工业（中英文）,2024,54(3):353-359,7.基金项目
2021年《化妆品安全技术规范》制修订项目 （）
2022年度浙江省药品监督管理局科技计划(编号:2021036) （编号:2021036）
