太原理工大学学报2024，Vol.55Issue(2)：384-389,6.DOI:10.16355/j.tyut.1007-9432.20230286
基底硬度介导IL-1β调控软骨细胞炎症响应
Substrate Stiffness Mediate Inflammatory Response of Chondrocyte Stimulated by IL-1β
摘要
Abstract
[Purposes]Osteoarthritis(OA)is a degenerative joint disease that commonly occurs in middle-aged and elderly people.Mechanical microenvironment is one of the most significant factors in the development of OA.However,when the mechanical microenvironment changes,the inflam-matory response of chondrocyte is elusive.[Methods]By adopting polydimethylsiloxane(PDMS)substrates with varying stiffness which can mimic the physiological stiffness of chondrocyte pericellu-lar matrix(PCM),influences of co-regulated substrate stiffness and inflammatory factors interleu-kin-1β(IL-1β)on chondrocyte morphology,inflammatory mediators,and PCM remodeling pro-tein expression are quantitatively analyzed.First,prostaglandin E2(PGE2)and nitric oxide(NO)released in different stiffness substrates and IL-1β stimulated substrates with chondrocytes are de-tected.Second,the changes in different stiffness substrates and IL-1β stimulated substrates through immunofluorescence technique are observed and recorded.Third,the protein expressions of type Ⅱcollagen(COLII)and matrix metalloproteinase-13(MMP13)are measured by Western blot assay.[Findings]The experimental results identify that substrate stiffness regulates the response of chon-drocyte to inflammatory signals.Soft substrate dramatically enhances the release of PGE2 and NO(P<0.000 1),and MMP13(P<0.05)expression,with IL-1β further enhances this regulation.In addition,stiff substrate significantly increases chondrocyte spreading area(P<0.000 1)and COLII expression level(P<0.01),IL-1β will further enhance this regulation.[Conclusions]This study will shed light into the mechanobiological mechanism of the chondrocyte sensing matrix me-chanical microenvironment,and provide a reference for optimizing cell-inductive biomaterials.关键词
软骨细胞/基底硬度/白细胞介素1β/细胞周基质/Ⅱ型胶原/基质金属蛋白酶13Key words
chondrocyte/substrate stiffness/interleukin-1β/pericellular matrix/type II collagen/matrix metalloproteinase-13分类
生物科学引用本文复制引用
孟楠,王笑笑,张艳君,陈维毅,卫小春,张全有..基底硬度介导IL-1β调控软骨细胞炎症响应[J].太原理工大学学报,2024,55(2):384-389,6.基金项目
国家自然科学基金资助项目(12272252,11872263) （12272252,11872263）
山西省基础研究计划资助项目(202203021211124) （202203021211124）
