武警医学2024，Vol.35Issue(3)：201-204,208,5.
髓源性生长因子对2型糖尿病小鼠胰岛素抵抗及肝脏炎性反应的影响
Effects of myeloid-derived growth factor on insulin resistance and inflammatory response of liver from type 2 diabetic mice
摘要
Abstract
Objective To investigate the effects of myeloid-derived growth factor(MYDGF)on insulin resistance and inflam-matory response of liver from type 2 diabetic mice.Methods C57 mice were randomly divided into Vehicle group,DM group and MYDGF group.After administration for 12 weeks,fasting blood glucose(FBG),insulin and HOMA-IR were determined.Tumor nec-rosis factor-α(TNF-α)and interleukin-6(IL-6)in serum as well as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and Catalase in the liver were measured.Morphological changes of liver were assessed by HE staining.Phosphorylation levels of IKKβ and IkBα were detected by Western blot.Results Compared with DM group,the levels of FBG[(6.91±0.71)mmol/L vs.(19.46±1.12)mmol/L],and insulin in MYDGF group[(13.29±1.42)Mu/L vs.(21.88±1.58)Mu/L]and HOMA-IR[(4.08±0.65)vs.(18.90±1.37)]obviously decreased(P＜0.05).The concentrations of TNF-α[(32.17±3.18)ng/L vs.(66.39±4.51)ng/L]and IL-6[(13.65±0.89)ng/L vs.(21.55±3.27)ng/L]also significantly decreased(P＜0.05).Liver SOD[(297.54±20.43)U/mg vs.(198.32±19.29)U/mg],GSH-Px[(0.65±0.05)U/mg vs.(0.19±0.04)U/mg]and Catalase[(220.34±19.82)U/mg vs.(134.69±18.81)U/mg]levels increased(P＜0.05);in addition,inflammatory cell infiltration and hepatocyte steatosis attenuated;lastly,phosphorylation levels of IKKβ[p-IKKβ:IKKβ,(0.97±0.10)vs.(1.39±0.11)]and IkBα[p-IκBα:IκBα,(0.93±0.07)vs.(1.44±0.06)]significantly increased(P＜0.05)in MYDGF group.Conclusions MYDGF im-proves insulin resistance,which may be attributed to attenuated inflammatory response from diabetic liver via activating the IKKβ/IkBα signaling pathway.关键词
髓源性生长因子/IKKβ/IkBα信号通路/2 型糖尿病/胰岛素抵抗Key words
myeloid-derived growth factor/IKKβ/IkBα sig-naling pathway/type 2 diabetes/insulin resistance分类
医药卫生引用本文复制引用
苏芳,付之光,方文灿,王莹,贺明娟,张永彬..髓源性生长因子对2型糖尿病小鼠胰岛素抵抗及肝脏炎性反应的影响[J].武警医学,2024,35(3):201-204,208,5.基金项目
武汉市知识创新专项项目(2022020801010556) （2022020801010556）
