髓源性生长因子对2型糖尿病小鼠胰岛素抵抗及肝脏炎性反应的影响OACSTPCD

Objective To investigate the effects of myeloid-derived growth factor(MYDGF)on insulin resistance and inflam-matory response of liver from type 2 diabetic mice.Methods C57 mice were randomly divided into Vehicle group,DM group and MYDGF group.After administration for 12 weeks,fasting blood glucose(FBG),insulin and HOMA-IR were determined.Tumor nec-rosis factor-α(TNF-α)and interleukin-6(IL-6)in serum as well as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and Catalase in the liver were measured.Morphological changes of liver were assessed by HE staining.Phosphorylation levels of IKKβ and IkBα were detected by Western blot.Results Compared with DM group,the levels of FBG[(6.91±0.71)mmol/L vs.(19.46±1.12)mmol/L],and insulin in MYDGF group[(13.29±1.42)Mu/L vs.(21.88±1.58)Mu/L]and HOMA-IR[(4.08±0.65)vs.(18.90±1.37)]obviously decreased(P＜0.05).The concentrations of TNF-α[(32.17±3.18)ng/L vs.(66.39±4.51)ng/L]and IL-6[(13.65±0.89)ng/L vs.(21.55±3.27)ng/L]also significantly decreased(P＜0.05).Liver SOD[(297.54±20.43)U/mg vs.(198.32±19.29)U/mg],GSH-Px[(0.65±0.05)U/mg vs.(0.19±0.04)U/mg]and Catalase[(220.34±19.82)U/mg vs.(134.69±18.81)U/mg]levels increased(P＜0.05);in addition,inflammatory cell infiltration and hepatocyte steatosis attenuated;lastly,phosphorylation levels of IKKβ[p-IKKβ:IKKβ,(0.97±0.10)vs.(1.39±0.11)]and IkBα[p-IκBα:IκBα,(0.93±0.07)vs.(1.44±0.06)]significantly increased(P＜0.05)in MYDGF group.Conclusions MYDGF im-proves insulin resistance,which may be attributed to attenuated inflammatory response from diabetic liver via activating the IKKβ/IkBα signaling pathway.