水凝胶创伤修复研究中应用的小型猪模型
Minipigs used in hydrogel wound repair research
摘要
Abstract
BACKGROUND:Minipigs are often used in research fields such as skin injury,vascular trauma and cosmetic medicine because they are highly similar to human beings in terms of skin tissue structure and cardiovascular system.Hydrogel as a wound repair drug possesses a variety of excellent physicochemical properties such as strong water retention and adhesion,which can provide isolation moisturization and drug release for wounds. OBJECTIVE:To summarize and conclude the progress of the application of trauma models for different experimental purposes of hydrogel therapy for minipigs,to reveal the development status of various types of minipig trauma models,to analyze the deficiencies of minipig trauma models at the present stage. METHODS:The relevant articles published in Web of Science database and CNKI database from the establishment of each database to 2023 were checked,using"piglet,miniature pig,minipig,miniature pig;gel,hydrogel;trauma,injury,wound,lesion,incision"as Chinese search terms and"Miniature Swine,Miniature pig,minipig;gel,hydrogel;injury,wound,lesion,incision"as English search terms.A total of 438 Chinese and English documents were retrieved,and 59 documents were included in the study through the inclusion and exclusion criteria. RESULTS AND CONCLUSION:(1)At present,the main models used clinically for trauma repair are large animal species(dogs and pigs),rabbits,and rodents(rats and mice).Because the skin structure of the minipig is more like that of humans,the minipig is the most ideal animal model for trauma repair.(2)In the in-vitro skin injury model,skin defect model is the basic wound model,which can be divided into full skin defect model and medium-thickness skin defect model according to the depth of the wound defect.Burn wound model and infected wound model are multidimensional models with hot metal scald and bacterial culture imposed on the basis of the skin defect model,which have the advantages of high safety coefficient and low operation difficulty.(3)In the in-vivo trauma repair model,mini-pigs are used as esophageal cricothyrotomy model which is more in line with the pathological state of clinical diseases.Mini-pigs are used in the gastric perforation and vascular hemostasis model,which can visually demonstrate the stronger organ adhesion,hemostatic properties and tissue regeneration-promoting effects of the hydrogel.(4)The specific parts of the pig also has the corresponding mode of use:pig ear is usually used to evaluate the hydrogel drug delayed-release effect.Porcine cellular proteins and pig skin collagen are mostly used to prepare composite hydrogels of tissue origin.关键词
小型猪/水凝胶/敷料/创伤模型/皮肤/综述Key words
minipig/hydrogel/dressings/trauma model/skin/review分类
医药卫生引用本文复制引用
梅日庚,耿少辉,林志敏,吴佳鹏,刘鑫,兰馨怡,高瑜若,黄光瑞..水凝胶创伤修复研究中应用的小型猪模型[J].中国组织工程研究,2024,28(29):4697-4702,6.基金项目
国防科技创新特区H863计划项目(20-163-02-ZT-008-003-01),项目参与人:黄光瑞 （20-163-02-ZT-008-003-01）
北京中医药大学大学生创新创业训练计划项目基金(s202310026032),项目负责人:林志敏 （s202310026032）
北京中医药大学大学生创新创业训练计划项目基金(X202310026070),项目参与人:林志敏 （X202310026070）
北京中医药大学大学生创新创业训练计划项目基金(X202310026158),项目参与人:刘鑫 National Defense Science and Technology Innovation Special Zone H863 Project,No.20-163-02-ZT-008-003-01(to HGR) （X202310026158）
Innovation and Entrepreneurship Training Program for College Students from Beijing University of Chinese Medicine,No.s202310026032(to LZM) （to LZM）
Innovation and Entrepreneurship Training Program for College Students from Beijing University of Chinese Medicine,No.X202310026070(to LZM) （to LZM）
Innovation and Entrepreneurship Training Program for College Students from Beijing University of Chinese Medicine,No.X202310026158(to LX) （to LX）
