现代畜牧科技Issue(3)：42-44,3.DOI:10.19369/j.cnki.2095-9737.2024.03.012
猫血型荧光PCR检测方法的建立及应用
Establishment and Application of Real-Time PCR for Detection of Feline Blood Type
赵方圆 1巩玉洁 1赵荣茂 1邓西 1毕铭钰1
作者信息
- 1. 北京纳百生物科技有限公司,北京 102600
- 折叠
摘要
Abstract
The AB blood type system in feline is composed of three blood types:A,B and AB.Mismatches may result in acute hemolytic transfusion reactions and neonatal isoerythrolysis.According to the principle of TaqMan probe fluorescence quantitative analysis.four sites of the feline blood type determining gene,cytidine-phospho-N-acetylneuraminic acid hydroxylase(CMAH)gene,were aligned,and the corresponding primers and probes were designed to establish a real-time PCR detection method for feline blood type.The real-time PCR method for feline blood type detection established in this study was 100％consistent with the results of Alvedia rapid-test(LabTest A+B).In addition.in the 269 feline samples collected in Beijing and surrounding areas,the proportions of A,B and AB blood type were 94.05％,5.58％and 0.37％,respectively.关键词
猫血型/CMAH基因/基因型检测/荧光PCRKey words
Feline blood type/CMAH gene/Detection of genotypes/real-time PCR分类
农业科技引用本文复制引用
赵方圆,巩玉洁,赵荣茂,邓西,毕铭钰..猫血型荧光PCR检测方法的建立及应用[J].现代畜牧科技,2024,(3):42-44,3.
