中国临床药理学杂志2024，Vol.40Issue(5)：688-692,5.DOI:10.13699/j.cnki.1001-6821.2024.05.012
水蛭对ApoE-/-动脉粥样硬化小鼠自噬的影响
Effect of Hirudo on autophagy in atherosclerotic mice
摘要
Abstract
Objective To explore the anti-atherosclerosis mechanism of Hirudo and its effect on autophagy in mice.Methods Forty healthy male ApoE-/-mice were randomly divided into model group,control group(3 × 10-3 g·kg-1·d-1 simvastatin)and experimental-L,experimental-M,experimental-H groups(0.45,0.9,1.8 g·kg-1· d-1,Maixuekang capsule).Eight healthy male C57BL/6J mice were divided into blank group.The mice were fed with common diet for 1 week.Then,except blank group,other groups were fed with high-fat diet.After 8 weeks of modeling,the atherosclerosis(AS)mice were given drugs orally once a day for 12 weeks,and fed with high-fat diet in the meantime.The levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)in serum were determined by enzyme-linked immunosorbent assay(ELISA).The levels of Beclin-1,LC3 autophagy protein were detected by Western blot method.Results The IL-6 contents in the experimental-H,experimental-M,experimental-L,control,model and blank groups were(107.59±3.03),(99.31±5.12),(103.52±2.28),(98.68±4.68),(112.66±6.08),(93.98±3.43)pg·mL-1;the TNF-α contents were(538.41±30.26),(504.49±21.51),(538.51±19.05),(494.05±25.08),(578.53±26.32),(467.35±21.53)pg·mL-1.For the above indexes,the differences between model group and experimental-H group,experimental-M group,experimental-L group,control group,blank group were all statistically significant(all P＜0.05).The Beclin-1 protein expression levels in the experimental-H,experimental-M,experimental-L,control and model groups were 1.48±0.05,1.72±0.05,1.19±0.02,1.51±0.04,0.66±0.03;the LC3 Ⅱ protein expression levels were 1.53±0.01,1.83±0.02,1.16±0.01,1.90±0.01,0.49±0.01,and the differences between model group and experimental-H group,experimental-M group,experimental-L group,control group were all statistically significant(all P＜0.05).Conclusion Hirudo can significantly reduce the area of atherosclerotic plaque by regulating the level of autophagy.关键词
水蛭/动脉粥样硬化/自噬/ApoE-/-小鼠/炎症因子/血脂Key words
Hirudo/atherosclerosis/autophagy/ApoE-/-mice/inflammatory factor/lipid分类
医药卫生引用本文复制引用
韩倩倩,潘芸芸,温紫云,吕晴,魏亮,李梦一,洪敏..水蛭对ApoE-/-动脉粥样硬化小鼠自噬的影响[J].中国临床药理学杂志,2024,40(5):688-692,5.基金项目
国家自然科学基金资助项目(82074139) （82074139）
广东省中医药管理局基金资助项目(20213013) （20213013）
