中国临床药理学杂志2024，Vol.40Issue(5)：693-697,5.DOI:10.13699/j.cnki.1001-6821.2024.05.013
柚皮苷对脓毒症引起的急性肺损伤小鼠保护作用的研究
Protective effect of naringin on sepsis-induced acute lung injury in mice
摘要
Abstract
Objective To investigate the effect and mechanism of naringin on acute lung injury(ALI)in septic mice.Methods The acute lung injury mouse model of sepsis was established by intraperitoneal injection of 10 mg·kg-1 lipopolysaccharide.The mice were randomly divided into control group(injected with equal amounts of saline and phosphate buffer),model group(mouse model of sepsis acute lung injury),naringin group(50 mg·kg-1 naringin injected intraperitoneally 1 hour prior to lipopolysaccharide modeling)and BzATP group(50 mg·kg-1 naringin+5 mg·kg-1 BzATP injected intraperitoneally 1 hour prior to lipopolysaccharide modeling).After modeling,lung tissues were taken 24 h later,lung coefficients were calculated;lung tissue interleukin(-ILβ-1 β),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)levels were detected by enzyme-linked immunosorbent assay;the expression of purinergic 2X7 receptor(P2X7R),nucleotide-binding oligomerization structural domain-like receptor protein 3(NLRP3)and nuclear factor-κB(NF-κB)proteins were detected by Western blotting.Results The lung coefficients in the control,model,naringin and BzATP groups were(6.26±0.31),(9.09±1.02),(7.02±0.45)and(8.79±0.55)mg·g-1;the contents of TNF-α were(56.41±0.35),(174.68±1.58),(85.23±1.68)and(162.97±3.42)pg·mL-1;the contents of IL-1β were(44.18±7.37),(119.91±17.16),(85.41±2.14)and(104.57±3.39)pg·mL-1;the contents of IL-10 were(50.82±2.89),(28.31±1.86),(42.82±1.98)and(25.19±1.69)pg·mL-1;P2X7 protein expression levels were 0.45±0.16,1.33±0.10,0.64±0.09 and 1.05±0.18;NF-κB protein expression levels were 0.38±0.19,1.29±0.09,0.57±0.11 and 0.92±0.07;NLRP3 protein expression levels were 0.72±0.14,1.28±0.23,0.75±0.09 and 1.27±0.23.Compared with the model group in the control group and naringin group,compared with the naringin group in the BzATP group,the differences of the above indexes were statistically significant(P＜0.01,P＜0.05).Conclusion Naringin attenuates acute lung injury in septic mice by inhibiting the P2X7 receptor-mediated NF-κB/NLRP3 signaling pathway.关键词
脓毒症/急性肺损伤/柚皮苷/嘌呤能2X7受体/核转录因子-KB/核苷酸结合寡聚化结构域样受体蛋白3Key words
sepsis/acute lung injury/naringin/purinergic 2X7 receptor/nuclear factor-κB/nucleotide-binding oligomerization domain-like receptors family pyrin domain containing 3分类
医药卫生引用本文复制引用
刘澈,马源,董旭鹏,段倩雯,雷娇,马玉清..柚皮苷对脓毒症引起的急性肺损伤小鼠保护作用的研究[J].中国临床药理学杂志,2024,40(5):693-697,5.基金项目
兰州大学第一医院院内基金资助项目(ldyyyn2022-5) （ldyyyn2022-5）
