柚皮苷对脓毒症引起的急性肺损伤小鼠保护作用的研究OA北大核心CSTPCD
Protective effect of naringin on sepsis-induced acute lung injury in mice
目的 研究柚皮苷对脓毒症小鼠急性肺损伤(ALI)的作用及机制.方法 通过腹腔注射10 mg·kg-1脂多糖建立脓毒症急性肺损伤小鼠模型.将小鼠随机分为对照组(注射等量磷酸缓冲液)、模型组(脓毒症急性肺损伤小鼠模型)、柚皮苷组(脂多糖造模前1 h腹腔注射50 mg·kg-1柚皮苷)、BzATP组(脂多糖造模前1 h腹腔注射50 mg·kg-1柚皮苷+5 mg·kg-1 BzATP).造模后24 h取肺组织,计算肺系数;用酶联免疫吸附实验检测肺组织白细胞介素-1β(IL-1β)、IL-10、肿瘤坏死因子-α(TNF-α)水平;用蛋白质印迹法检测肺组织嘌呤能2X7受体(P2X7R)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、核转录因子-κB(NF-κB)蛋白的表达水平.结果 对照组、模型组、柚皮苷组和 BzATP 组的肺系数分别为(6.26±0.31)、(9.09±1.02)、(7.02±0.45)和(8.79±0.55)mg·g-1;TNF-α 含量分别为(56.41±0.35)、(174.68±1.58)、(85.23±1.68)和(162.97±3.42)pg·mL-1;IL-1β 含量分别为(44.18±7.37)、(119.91±17.16)、(85.41±2.14)和(104.57±3.39)pg·mL-1;IL-10 含量分别为(50.82±2.89)、(28.31±1.86)、(42.82±1.98)和(25.19±1.69)pg·mL-1;P2X7R 表达水平分别为 0.45±0.16、1.33 士 0.10、0.64±0.09 和 1.05±0.18;NF-κB 表达水平分别为 0.38±0.19、1.29±0.09、0.57±0.11 和 0.92±0.07;NLRP3 表达水平分别为 0.72±0.14、1.28±0.23、0.75±0.09和1.27±0.23.对照组、柚皮苷组上述指标与模型组比较,BzATP组上述指标与柚皮苷组比较,在统计学上差异均有统计学意义(P＜0.01,P＜0.05).结论 柚皮苷可通过抑制P2X7受体介导NF-KB/NLRP3信号通路,减轻脓毒症小鼠急性肺损伤.
Objective To investigate the effect and mechanism of naringin on acute lung injury(ALI)in septic mice.Methods The acute lung injury mouse model of sepsis was established by intraperitoneal injection of 10 mg·kg-1 lipopolysaccharide.The mice were randomly divided into control group(injected with equal amounts of saline and phosphate buffer),model group(mouse model of sepsis acute lung injury),naringin group(50 mg·kg-1 naringin injected intraperitoneally 1 hour prior to lipopolysaccharide modeling)and BzATP group(50 mg·kg-1 naringin+5 mg·kg-1 BzATP injected intraperitoneally 1 hour prior to lipopolysaccharide modeling).After modeling,lung tissues were taken 24 h later,lung coefficients were calculated;lung tissue interleukin(-ILβ-1 β),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)levels were detected by enzyme-linked immunosorbent assay;the expression of purinergic 2X7 receptor(P2X7R),nucleotide-binding oligomerization structural domain-like receptor protein 3(NLRP3)and nuclear factor-κB(NF-κB)proteins were detected by Western blotting.Results The lung coefficients in the control,model,naringin and BzATP groups were(6.26±0.31),(9.09±1.02),(7.02±0.45)and(8.79±0.55)mg·g-1;the contents of TNF-α were(56.41±0.35),(174.68±1.58),(85.23±1.68)and(162.97±3.42)pg·mL-1;the contents of IL-1β were(44.18±7.37),(119.91±17.16),(85.41±2.14)and(104.57±3.39)pg·mL-1;the contents of IL-10 were(50.82±2.89),(28.31±1.86),(42.82±1.98)and(25.19±1.69)pg·mL-1;P2X7 protein expression levels were 0.45±0.16,1.33±0.10,0.64±0.09 and 1.05±0.18;NF-κB protein expression levels were 0.38±0.19,1.29±0.09,0.57±0.11 and 0.92±0.07;NLRP3 protein expression levels were 0.72±0.14,1.28±0.23,0.75±0.09 and 1.27±0.23.Compared with the model group in the control group and naringin group,compared with the naringin group in the BzATP group,the differences of the above indexes were statistically significant(P＜0.01,P＜0.05).Conclusion Naringin attenuates acute lung injury in septic mice by inhibiting the P2X7 receptor-mediated NF-κB/NLRP3 signaling pathway.
刘澈;马源;董旭鹏;段倩雯;雷娇;马玉清
兰州大学第一临床医学院,甘肃兰州 730000兰州大学第一医院麻醉科,甘肃兰州 730000
中医学
脓毒症急性肺损伤柚皮苷嘌呤能2X7受体核转录因子-KB核苷酸结合寡聚化结构域样受体蛋白3
sepsisacute lung injurynaringinpurinergic 2X7 receptornuclear factor-κBnucleotide-binding oligomerization domain-like receptors family pyrin domain containing 3
《中国临床药理学杂志》 2024 (005)
693-697 / 5
兰州大学第一医院院内基金资助项目(ldyyyn2022-5)
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