中国临床药理学杂志2024，Vol.40Issue(5)：698-702,5.DOI:10.13699/j.cnki.1001-6821.2024.05.014
意大利牛舌草对咳嗽变异性哮喘大鼠TLR4/NF-κB信号通路的影响
Effects of Anchusa italica Retz.on cough variant asthma and the level of TLR4/NF-κB signaling pathway
摘要
Abstract
Objective To investigate the effect of Anchusa italica Retz.extract on cough variant asthma(CVA)and its mechanism of action.Methods Forty-eight SD rats of each eight were randomly divided into blank group(equal amount of saline),model group(equal amount of saline),control group(250 mg·kg-1 prednisone acetate)and experimental-A1,-A5,-A7 groups(61.1 mg·kg-1 Anchusa italica Retz.extract A1,48.8 mg·kg-1 Anchusa italica Retz.extract A5,201.8 mg·kg-1 Anchusa italica Retz.extract A7),continuous gavage for 28 d;except for the blank group,the other groups were made with ovalbumin-complete Freund's adjuvant.The number of cough times of each rat was observed.The changes in the expression of Toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88)and nuclear factor κB(NF-κB)proteins were observed in the lung tissue.Results The cough times in the blank group,model group,control group and experimental-A1,-A5,-A7 groups were 1.10±0.22,8.33±1.24,3.08±0.65,3.31±0.99,3.08±1.02 and 3.06±0.68;the relative expression levels of TLR4 protein were 0.61±0.01,0.84±0.04,0.66±0.02,0.64±0.04,0.64±0.03 and 0.69±0.02;the relative expression levels of MyD88 protein were 0.54±0.08,0.86±0.06,0.71±0.06,0.65±0.05,0.64±0.08 and 0.70±0.06;the relative expression levels of p-NF-κB protein were 0.48±0.11,0.94±0.06,0.80±0.08,0.68±0.04,0.68±0.06 and 0.78±0.09.Compared with the experimental-A1,-A5,-A7 groups,control group,normal group,the differences were statistically significant in the model group(all P＜0.05).Conclusion The extract of Anchusa Italica Retz.can inhibit the symptoms of CVA rats,and the mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signaling pathway.关键词
意大利牛舌草/咳嗽变异性哮喘/Toll样受体-4/核因子κBKey words
Anchusa Italica Retz./cough variant asthma/Toll-like receptor/nuclear factor-kappa B分类
医药卫生引用本文复制引用
陈良,徐晓琴,郭文辉,阿吉艾克拜尔·艾萨,买迪娜..意大利牛舌草对咳嗽变异性哮喘大鼠TLR4/NF-κB信号通路的影响[J].中国临床药理学杂志,2024,40(5):698-702,5.基金项目
新疆维吾尔自治区自然科学基金项目联合基金面上项目(2021D01C222) （2021D01C222）
新疆医科大学省部共建中亚高发病成因与防治国家重点实验室开放课题基金资助项目(SKL-HIDCA-2020-ZY12) （SKL-HIDCA-2020-ZY12）
新疆地产中药民族药新药研发培育基金资助项目(2017-02-08) （2017-02-08）
