中国临床药理学杂志2024，Vol.40Issue(5)：728-731,4.DOI:10.13699/j.cnki.1001-6821.2024.05.020
用UPLC-MS/MS法测定人血浆中厄他培南的浓度
Determination of ertapenem in human plasma by UPLC-MS/MS
陶依然 1刘博宇 1胡蕾 1刘思鲁 1赵海艳 1薛学财 1黄琳1
作者信息
- 1. 北京大学人民医院药学部,北京 100044
- 折叠
摘要
Abstract
Objective A simple,sensitive and rapid ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was developed and validated for the determination of ertapenem in human plasma.Methods Using ertapenem-D4 as internal standard,the protein in plasma was precipitated with acetonitrile;chromatographic column:ACQUITY HSS T3(2.1 mm × 50.0 mm,1.8 μm);the mobile phase was 0.1％formic acid aqueous solution(containing 2 mmol·L-1 ammonium formate)-acetonitrile(0.1％formic acid),using a gradient elution;flow rate:0.4 mL·min-1,injection volume:1 μL,column temperature:45 ℃,the analysis time was 4.5 min,the scanning mode is positive ion selective reaction monitoring mode(SRM)with an electric spray ion source(ESI).The specificity,standard curve and lower limit of quantification,precision and recovery,matrix effect,dilution effect and stability were investigated.Results Ertapenem had a good linearity within 0.5-80.0μg·mL-1,and the standard curve was y=4.25 × 10-1x-2.64× 10-2(r2=0.999 0),the lower limit of quantification was 0.5 μg·mL-1,the relative standard deviation within and between batches is 1.39％-4.15％.The extraction recovery rate was 58.36％-64.57％,and the relative standard deviation of dilution effect was 3.30％,and the matrix effect was 99.71％-103.23％.The relative standard deviation of room temperature,repeated freeze-thaw,4 ℃,and long-term stability are all less than 10％.Conclusion The method is sensitive,rapid and specific,which is suitable for clinical monitoring of Ertapenem.关键词
厄他培南/液质联用/治疗药物监测Key words
ertapenem/liquid chromatography-tandem mass spectrometry/therapeutic drug monitoring分类
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陶依然,刘博宇,胡蕾,刘思鲁,赵海艳,薛学财,黄琳..用UPLC-MS/MS法测定人血浆中厄他培南的浓度[J].中国临床药理学杂志,2024,40(5):728-731,4.
