中国司法鉴定Issue(1)：53-60,8.DOI:10.3969/j.issn.1671-2072.2024.01.008
超高效液相色谱-串联质谱法同时测定人血中12种抗抑郁药
Simultaneous Determination of 12 Antidepressants in Human Blood by UHPLC-MS/MS
徐峰 1宣宇 2卓晓聪 1富利祥 1陈峰 1王斌杰 3郭狄飞 4余鹏飞5
作者信息
- 1. 杭州市公安局萧山区分局 刑事科学技术室,浙江 杭州 311200
- 2. 浙江省公安厅刑侦总队 浙江省刑事科学技术应用研究重点实验室,浙江 杭州 310009
- 3. 浙江警察学院 刑事科学技术系,浙江 杭州 310053
- 4. 杭州海康威视电子有限公司质量检测中心,浙江 杭州 311500
- 5. 绿城农科检测技术有限公司,浙江 杭州 310052
- 折叠
摘要
Abstract
Objective To establish an analytical method for the simultaneous determination of 12 antidepressants including moclobemide and bupropion in human blood by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Methods Blood samples were purified through protein precipitation with acetonitrile that contained internal standard(proadifen),then the samples were injected into the UHPLC-MS/MS.The separation was performed on an ACQUITY UPLC HSS T3 column(2.1 mm×100 mm,1.8 µm)using gradient elution with a flow rate of 0.35 mL/min.The mobile phase consisted of water solution and acetonitrile solution that both contained 2 mmol/mL ammonium formate and 0.1%formic acid.The analytes were detected using multiple reaction monitoring mode.The electrospray ionization source was performed in positive mode.The internal standard method was used for quantification.Results The correlation coefficients of the 12 antidepressants were better than 0.999 in the corresponding concentration ranges,and the limits of detection ranged from 0.05 ng/mL to 0.2 ng/mL,the limits of quantification ranged from 0.5 ng/mL to 1 ng/mL.At different spiked concentrations(low,medium and high),the accuracy ranged from-7.1%to 11.7%,the intra-day precision ranged from 0.1%to 11.5%,the inter-day precision ranged from 1.9%to 9.4%,the extraction recoveries ranged from 85.7%to 113.5%,and the matrix effect ranged from-18.2%to 10.2%.The stability of each component at different levels of concentration and under different environment conditions ranged from-9.30%to 11.30%.All of the above results meet the requirements for biological sample analysis and forensic toxicology analysis.Conclusion The method is selective,reproducible and precise.The simultaneous determination of 12 antidepressants in human blood can be applied in forensic toxicology practice and it will be of great use in the analysis of the cause of death in unnatural death cases.关键词
法医毒物学/超高效液相色谱-串联质谱法/抗抑郁药/人血Key words
forensic toxicology/ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)/antidepressant/human blood分类
社会科学引用本文复制引用
徐峰,宣宇,卓晓聪,富利祥,陈峰,王斌杰,郭狄飞,余鹏飞..超高效液相色谱-串联质谱法同时测定人血中12种抗抑郁药[J].中国司法鉴定,2024,(1):53-60,8.
