中国司法鉴定Issue(1)：61-65,5.DOI:10.3969/j.issn.1671-2072.2024.01.009
基于线粒体DNA和核DNA遗传标记的中华鲟种属鉴定
Species Identification of Acipenser sinensis Based on Mitochondrial DNA and Nuclear DNA Genetic Markers
摘要
Abstract
Objective To establish a method for the identification of Acipenser sinensis and its potential hybrids based on mitochondrial DNA and nuclear DNA markers.Methods Primers were designed according to the cytb gene sequence of Acipenser sinensis published in NCBI,and the single-copy nuclear gene fam43a amplification primers of sturgeon were used to amplify and sequence the DNA of Acipenser sinensis.The amplified product of fam43a gene was TA cloned and sequenced.The source of the sample was determined by BLAST sequence similarity search,and the phylogenetic tree of Acipenseridae was constructed based on the neighbor-joining method.Results The lengths of cytb and fam43a gene sequences obtained by sequencing the Acipenser sinensis samples were 728 bp and 730 bp,respectively.The fam43a gene sequences of the 10 clonal populations were completely consistent.The analysis of BLAST sequence similarity search showed that the measured cytb and fam43a gene sequences had the highest similarity with the Acipenser sinensis in the GenBank database,which were 99.86%and 100%,respectively.Phylogenetic analysis showed that the sequences of Acipenser sinensis all clustered with the DNA sequences of Acipenser sinensis in the GenBank database,and belonged to different clades with other species of Acipenseridae.Conclusion The combined use of mitochondrial DNA and nuclear DNA markers can quickly and accurately identify Acipenser sinensis and its potential hybrids.This method can meet the needs of forensic identification cases and provide technical support for the rescue and protection of Acipenser sinensis.关键词
法医遗传学/cytb基因/fam43a基因/中华鲟/种属鉴定Key words
forensic genetics/cytb gene/fam43a gene/Acipenser sinensis/species identification分类
社会科学引用本文复制引用
杨子豪,夏若成,蒋志伟,蒋婷婷,陈开琴,吴晓连,陶瑞旸,杜志淳..基于线粒体DNA和核DNA遗传标记的中华鲟种属鉴定[J].中国司法鉴定,2024,(1):61-65,5.基金项目
国家自然科学基金项目(81930056) （81930056）
上海市法医学重点实验室资助项目(21DZ2270800) （21DZ2270800）
上海市司法鉴定专业技术服务平台资助项目. （）
