肺动脉高压大鼠模型中PKM2 Neddylation修饰促进右心室纤维化的研究OACSTPCD
Effect of PKM2 Neddylation Modification on Right Ventricular Fibro-sis in Pulmonary Hypertension Rats
目的 探究PKM2 拟素化(neddylation)修饰对肺动脉高压大鼠右心室纤维化的影响.方法 将SD大鼠随机分为对照组、模型组和MLN4924 组,HE染色法检测肺动脉,Masson染色检测右心室纤维化程度.提取大鼠原代心脏成纤维细胞,免疫荧光法检测α-平滑肌肌动蛋白(α-SMA)表达,使用si-NC、si-PKM2、pcDNA3.1-PKM2 转染原代心脏成纤维细胞后,蛋白质免疫印迹法检测PKM2 及纤维化相关Ⅰ型胶原蛋白(Collagen…查看全部>>
Objective Exploring the effect of PKM2 neddylation modification on myocardial fi-brosis in pulmonary hypertension rats.Methods SD rats were randomly divided into 3 groups:con-trol group,model group,and MLN4924 group.Pulmonary arteries were detected by HE staining,and right ventricular fibrosis was detected by Masson staining.Primary cardiac fibroblasts were isola-ted from rats and the expression of α-Smooth muscle actin(α-SMA)was measured by immunofluo-resce…查看全部>>
郭文昀;王丽霞;王金琳
联勤保障部队第940医院心血管内科 兰州市,730050联勤保障部队第940医院心血管内科 兰州市,730050联勤保障部队第940医院心血管内科 兰州市,730050
基础医学
肺动脉高压PKM2拟素化修饰右心室纤维化
pulmonary arterial hypertensionPKM2neddylationright ventricular fibrosis
《医学分子生物学杂志》 2024 (2)
108-114,7
甘肃省自然科学基金(No.22JR5RA012),联勤保障部队第940 医院院内项目(No.2021yxky080) This work was supported by grants from the Natural Science Foundation of Gansu Province(No.22JR5RA012),the Project of Joint Support Force 940 Hospital(No.2021yxky080)
评论