医学分子生物学杂志2024，Vol.21Issue(2)：129-134,6.DOI:10.3870/j.issn.1672-8009.2024.02.006
中性粒细胞胞外诱捕网通过CCDC25促进骨肉瘤细胞增殖、迁移和侵袭的作用研究
Role of CCDC25 Mediated Neutrophil Extracellular Traps in Promo-ting Proliferation,Migration and Invasion of Osteosarcoma Cells
摘要
Abstract
Objective To investigate the role and underlying mechanisms of neutrophil extra-cellular traps(NETs)in the proliferation,migration,and invasion of osteosarcoma cells.Methods A total of 20 osteosarcoma patients and 20 healthy subjects were enrolled.Levels of PAD4 and H3cit in serum were measured by ELISA.Neutrophils were isolated from peripheral blood of both groups to compare NETs formation.Co-culturing experiments were conducted by incubating NETs with osteosarcoma cells(MG63),with the addition of DNA degrading enzyme DNase Ⅰ.The groups included Control,NETs,and NETs + DNase Ⅰ groups.CCDC25 protein expression level was detected using Western blotting,cell proliferation was assessed using CCK-8 assay,and cell migration and invasion were evaluated using Transwell assay.MG63 cells were transfected with si-NC and si-CCDC25,then co-cultured with NETs,cells were then divided into 2 groups:si-NC and si-CCDC25 groups.CCDC25 protein expression,cell proliferation,and invasion were assessed as de-scribed above.Results Compared to healthy subjects,osteosarcoma patients showed elevated lev-els of PAD4 and H3cit in serum(P<0.05),indicating enhanced NETs formation capaci-ty.Compared to the Control group,the NETs group exhibited elevated CCDC25 protein expression level,increased cell proliferation and cell migration and invasion(P<0.05).The NETs + DNase Ⅰ group showed reduced CCDC25 protein expression level,decreased cell proliferation,cell mi-gration and invasion compared to the NETs group(P<0.05).The si-CCDC25 group,when com-pared to the si-NC group,demonstrated decreased CCDC25 protein expression level,cell prolifera-tion,cell migration and invasion(P<0.05).Conclusion NETs activate osteosarcoma cell CCDC25 expression through released DNA,thereby promoting tumor cell proliferation,and cell mi-gration and invasion.关键词
中性粒细胞胞外诱捕网/骨肉瘤/脱氧核糖核酸酶Ⅰ/卷曲螺旋结构域蛋白25Key words
neutrophil extracellular traps/osteosarcoma/deoxyribonuclease Ⅰ/coiled-coil domain-containing protein 25分类
医药卫生引用本文复制引用
木特力·买买提,徐磊磊,李文强,颜飞华..中性粒细胞胞外诱捕网通过CCDC25促进骨肉瘤细胞增殖、迁移和侵袭的作用研究[J].医学分子生物学杂志,2024,21(2):129-134,6.基金项目
新疆维吾尔自治区自然科学基金(No.2021D01C335) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2021D01C335) （No.2021D01C335）
