医学分子生物学杂志2024，Vol.21Issue(2)：135-140,6.DOI:10.3870/j.issn.1672-8009.2024.02.007
舒芬太尼通过长链非编码RNA CCAT1调控宫颈癌细胞周期和凋亡
Effect of Sufentanil on Cervical Cancer Cell Cycle and Apoptosis Through Long Non-coding RNA CCAT1
摘要
Abstract
Objective To investigate the molecular mechanisms by which sufentanil affects cer-vical cancer(CC)cells.Methods Real-time quantitative PCR(RT-qPCR)assay was used to detect the expression level of lncRNA CCAT1 in CC tissues and precancerous tissues,and in the cervical epithelial cells GH329 and CC cell lines(HeLa,CaSki,Siha and C4-1).Subsequently,CC cells CaSki were randomly divided into 4 groups:blank group,sufentanil group,sufentanil + oe-NC group and sufentanil + CCAT1 group.RT-qPCR assay was then used to examine the expres-sion level of lncRNA CCAT1 in each group.Cell Counting Kit-8(CCK-8)assay was used to detect the cell proliferation ability.Flow cytometry was used to detect the changes of cell cycle and the cell apoptosis.Results lncRNA CCAT1 was highly expressed in CC tissues and the CC cell lines(He-La,CaSki,Siha and C4-1)when compared with that in the precancerous tissues and that in the GH329 cells respectively(P<0.01).The expression level of lncRNA CCAT1 in and the prolifera-tion ability of CaSki cells were significantly decreased(P<0.01),while the proportion of cells in the G0/G1 phase and the apoptosis rate were significantly increased(P<0.01)in the sufentanil group when compared with those in the blank group.The expression level of lncRNA CCAT1 and the prolifera-tion ability of CaSki cells in the sufentanil +oe-CCAT1 group were significantly increased(P<0.01),while the proportion cells in the G0/G1 phase and cell apoptosis rate were significantly decreased(P<0.01)when compared with those in the sufentanil +oe-NC group.Conclusion Sufentanil could block the CC cell cycle by decreasing lncRNA CCAT1 expression in CC cells,ultimately inhibiting the prolif-erative ability of CC cells and promoting cell apoptosis.关键词
宫颈癌/舒芬太尼/lncRNA CCAT1/细胞周期/增殖活力/细胞凋亡Key words
cervical cancer/sufentanil/lncRNA CCAT1/cell cycle/proliferation viability/apoptosis分类
医药卫生引用本文复制引用
温旭,庞小翼,文艳,王蓉,杜倩,肖松..舒芬太尼通过长链非编码RNA CCAT1调控宫颈癌细胞周期和凋亡[J].医学分子生物学杂志,2024,21(2):135-140,6.基金项目
四川省医学(青年创新)科研课题(No.S20029) This work was supported by a grant from the Sichuan Medical(Youth innovation)Research Project(No.S20029) （青年创新）
