医学分子生物学杂志2024，Vol.21Issue(2)：141-145,153,6.DOI:10.3870/j.issn.1672-8009.2024.02.008
番泻苷B通过Wnt/β-catenin通路抑制视网膜母细胞瘤HXO-Rb44细胞增殖、凋亡和侵袭
Sennoside B Inhibits the Proliferation,Apoptosis and Invasion of Ret-inoblastoma HXO-Rb44 Cells via Wnt/β-catenin Pathway
摘要
Abstract
Objective To investigate the effect of sennoside B on proliferation,apoptosis,in-vasion and Wnt/β-catenin signaling pathway of retinoblastoma HXO-Rb44 cells.Methods HXO-RB44 cells were treated with different doses(0,5,10 and 20 μmol/L)of sennoside B,and the cells were randomly divided into four groups:Control group,sennoside B 5 μmol/L group,senno-side B 10 μmol/L group and sennoside B 20 μmol/L group.Cell viability was detected by MTT as-say.Colony formation assay was used to detect the colony formation rate.Cell apoptosis rate was de-tected by flow cytometry.Cell invasion were detected by Transwell assay.Cell pellet-forming experi-ment was used to detect the diameters and numbers of cell pellets.The protein expression levels of Cleaved Caspase-3,Caspase-3,MMP-2,MMP-9,SOX2,OCT4,CD44,Wnt1,β-catenin were detected by Western blotting.Results The cell viabilities and colony formation rates in the sennoside B 10 and 20 μmol/L groups were significantly decreased when compared with those in the Control group(P<0.05),the cell apoptosis rates and cleaved Caspase-3/Caspase-3 expression levels were significantly increased when compared with those in the Control group(P<0.05).The numbers of invaded cells and the protein expression levels of MMP-2 and MMP-9 were significantly decreased in the sennoside B 10 and 20 μmol/L groups(P<0.05),the cell pellet diameters,the cell pellet numbers and the protein expression levels of SOX2,OCT4 and CD44 were significantly decreased(P<0.05),and the protein expression levels of Wnt1 and β-catenin were significantly decreased in the sennoside B 10 and 20 μmol/L groups when compared with those in the Control group(P<0.05).Conclusion Sennoside B can induce apoptosis,inhibit the proliferation,inva-sion and stem-like characteristics of retinoblastoma HXO-Rb44 cells,and inhibit the activation of Wnt/β-catenin signaling pathway.关键词
视网膜母细胞瘤/番泻苷B/干细胞样特性/Wnt/β-catenin信号通路Key words
retinoblastoma/sennoside B/stem cell-like properties/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
孙蒙蒙,崔博坤,贾梦,冉柳,王丹荣,冯素婷,张虎,郝建章..番泻苷B通过Wnt/β-catenin通路抑制视网膜母细胞瘤HXO-Rb44细胞增殖、凋亡和侵袭[J].医学分子生物学杂志,2024,21(2):141-145,153,6.基金项目
河北省重点研发计划项目(No.223777105D),保定市科技计划项目(No.2041ZF240) This work was supported by grants from the Key R&D Plan Projects in Hebei Province(No.223777105D)and Baoding Science and Technology Plan Project(No.2041ZF240) （No.223777105D）
