南方农业学报2023，Vol.54Issue(12)：3475-3487,13.DOI:10.3969/j.issn.2095-1191.2023.12.003
宁春4号×河东乌麦F6:7家系籽粒蛋白质性状及候选基因分析
Grain protein traits and candidate genes analysis in F6:7 pedigrees derived from Ningchun No.4×Hedong black wheat
摘要
Abstract
[Objective]Analysis of grain protein traits and candidate genes in F6:7 pedigrees of Ningchun No.4 and He-dong black wheat through the cross and self-cross was carried out to discover candidate genes associated with grain pro-tein traits,which will provide theoretical reference for genetic improvement of grain protein traits in wheat.[Method]551 F6:7 pedigrees deriving from the cross and self-cross between two parents(Ningchun No.4 and Hedong black wheat)which showed significant differences in grain protein traits,were used as materials.Variance analysis,correlation analysis and cluster analysis were performed to analyze the grain protein traits(crude protein content,wet gluten content and sedi-mentation value)and transcriptome sequencing on high and low value strains,functional annotation,signal pathway enrichment analysis,and identification of genes related to grain protein traits were carried out on differentially expressed genes(DEGs).[Result]The grain protein-related traits in the F6:7 pedigrees were obviously separated,and the distribution was continuous normal distribution.The average values of crude protein content,wet gluten content and sedimentation value were 15.64％,33.31％and 39.06 mL,respectively,between the high and low parent strains.The proportions of pedi-grees exceeding the mid-parent value ranged from 29.04％to 77.31％,and the proportions of pedigrees exceeding the high-parent value ranged from 7.08％to 39.56％.The order of variation coefficient was sedimentation value(10.99％)>wet gluten content(6.07％)>crude protein content(5.70％),and the three traits showed extremely significant positive correla-tion(P<0.001).The 551 F6:7 pedigrees were clustered into four groups(Ⅰ～Ⅳ),including 148,125,171 and 107 materi-als,respectively.Group IV displayed the highest average crude protein content(16.87％),wet gluten content(35.96％)and sedimentation value(45.02 mL),making it the superior group.According to the transcriptome sequencing data of high and low value strains screened out 4368 DEGs,in which were 970 upregulated genes and 3398 downregulated genes.GO functional annotation results showed that the differential expression of wheat protein traits mainly occurring in the cellular part,involving membrane structures,organelles,and cell connections,the coordinated variation of serial biological processes occurred by regulating binding and catalytic molecular functions including cellular process,metabolic process,defense response and response to stimulus pathways.KEGG signaling pathway enrichment results showed that the DEGs were significantly(P<0.05)enriched into nine metabolism pathways,four metabolism pathways screened and their enriched DEGs were closely related to wheat grain protein traits,10 DEGs were related to histidine metabolism,21 related to starch and sucrose metabolism,8 related to tyrosine metabolism,8 related to alanine,aspartate and glutamate metabo-lism.In them,there were 7 upregulated expression genes.The results of real-time quantitative PCR verification were basi-cally consistent with the RNA-Seq results.[Conclusion]The grain protein traits belong to quantitative trait inheritance controlled by multiple genes in wheat,and many super-parents types were appeared in F6:7 pedigrees.The variation coeffi-cient of sedimentation value had greater potential for improvement.Group IV was superior group in grain protein traits and could be used in the breeding practices of varieties with superior protein traits.Four pathways were screened,includ-ing histidine metabolism,starch and sucrose metabolism,tyrosine metabolism,and alanine,aspartate and glutamate metabolism based on enrichment functional analysis,were related to the regulation mechanism of wheat grain protein traits.7 candidate genes were identified possibly related to wheat grain protein traits.关键词
小麦/家系/粗蛋白含量/湿面筋含量/沉降值/转录组Key words
wheat/pedigree/crude protein content/wet gluten content/sedimentation value/RNA-Seq分类
农业科技引用本文复制引用
陈佳静,王掌军,毛馨缘,王新杰,李清峰,刘彩霞,张雪婷,赵补全,亢玲,李前荣..宁春4号×河东乌麦F6:7家系籽粒蛋白质性状及候选基因分析[J].南方农业学报,2023,54(12):3475-3487,13.基金项目
国家自然科学基金项目(32160452) （32160452）
宁夏自然科学基金项目(2023AAC03060) Natural Science Foundation of Ningxia Hui Autonomous Region(2023AAC03060) （2023AAC03060）
National Natural Science Foundation of China(32160452) （32160452）
