南方农业学报2023，Vol.54Issue(12)：3581-3590,10.DOI:10.3969/j.issn.2095-1191.2023.12.012
三氟苯嘧啶对灰飞虱的活性及代谢酶和保护酶活性的影响
The difference bioactivities of triflumezopyrim and effect of tri-flumezopyrim on protective and detoxification enzyme activities to Laodelphax striatellus
摘要
Abstract
[Objective]In order to provide useful information in the application of trifluorophenyl for the management of L.striatellus in the field.The different sensitivities of six Laodelphax striatellus Fallén populations collected from in Shandong,China to trifluorophenyl(a new mesoionic insecticide)were detected.The effects of the sublethal doses(LC10 and LC30)of trifluorophenyl on the activities of protective and detoxifying enzymes in adults of L.striatellus were also as-sessed.[Method]The bioactivities of trifluorophenyl to the 3rd instar nymphs and adults of six populations collected from'Daiyue,Laiwu,Changqing,Jiyang and Yutai(from wheat and rice,respectively),was evaluated by rice seedlings dipping method and topical application method in the laboratory,respectively.The sublethal effects(LC10 and LC30)of trifluoro-phenyl on the activities of three protective enzymes[superoxide dismutase(SOD)、peroxidase(POD)and catalase(CAT)]and three detoxifying enzymes[glutathione S-transferase(GSTs)、carboxylesterase(CarE)and cytochrome P450(P450)]in 3rd instar nymphs of L.striatellus tby using seedlings dipping method were also measured.[Result]The results showed that the LD50 values of trifluorophenyl to 3rd instar nymphs of six populations of L.striatellus ranged from 3.487×10-4 µg/insect to 7.441×10-4 µg/insect and to adults from 1.947×10-4µg/insect to 4.196×10-4µg/insect by using topi-cal application method.The LC50 values of trifluorophenyl to 3rd instar nymphs of six populations of L.striatellus ranged from 0.756 mg/L to 1.523 mg/L and to adults from 0.752 mg/L t to 1.076 mg/L by using rice seedlings dipping method.Af-ter 72 h of treatment with two sublethal doses of trifluoropyrimidine,compared with the control group,the activities of POD,SOD,GSTs and P450 in L.striatellus adults were significantly increased,while activities of CarE were significantly inhibited,and showed obvious dose effect.The activities of CAT were increased in low concentrations(LC10)treatment and decreased in high concentrations(LC30)treatment,respectively.[Conclusion]The results indicated that trifluorophe-nyl has high insecticidal activities in contact and stomach toxicity against L.striatellus.The sensitivity of six L.striatellus populations to trifluoropyrimidine was higher,and no sensitive differences in different areas of Shandong province.The protective(POD and SOD)and detoxifying(GSTs and P450)enzymes played important roles in the detoxification and resistance of of L.striatellus to trifluoropyrimidine.These results can be used as the relative sensitivity baseline of L.stri-atellus to trifluoropyrimidine in Shandong province,and will help to provide the valuable information for the application of trifluoropyrimidine in the field.关键词
三氟苯嘧啶/灰飞虱/敏感性/亚致死剂量/保护酶/解毒酶Key words
Trifluorophenyl/Laodelphax striatellus/Sensitivity/Sublethal dose/Protective enzymes,Detoxifying enzymes分类
农业科技引用本文复制引用
丁文娟,杜若诗,李召阁,刘冬梅,刘畅,薛燕楠,曹俊丽,夏晓明..三氟苯嘧啶对灰飞虱的活性及代谢酶和保护酶活性的影响[J].南方农业学报,2023,54(12):3581-3590,10.基金项目
国家自然科学基金项目(32072459) National Natural Science Foundation of China(32072459) （32072459）
